蒙古黄芪苗期立枯病害及其病原菌鉴定  

Identification of Rhizoctonia solani and Its Pathogen in Astragalus membranaceus var.mongholicus Seedling Stage

作　　者：陈爱昌 王艳霞 漆永红[3] 罗宁 李惠霞 Chen Aichang;Wang Yanxia;Qi Yonghong;Luo Ning;Li Huixia(Dingxi Station of Plant Protection and Quarantine,Dingxi 743000,Gansu,China;Dingxi Station of Rural Energy,Dingxi 743000,Gansu,China;Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China;College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]定西市植保植检站,甘肃定西743000 [2]定西市农村能源站,甘肃定西743000 [3]甘肃省农业科学院植物保护研究所,兰州730070 [4]甘肃农业大学植物保护学院,兰州730070

出　　处：《农学学报》2021年第9期11-14,共4页Journal of Agriculture

基　　金：甘肃省农业科学院科研条件建设及成果转化项目“黄芩根腐病害防控关键技术研究”(2020GAAS25);国家重点研发计划“特色经济作物化肥农药减施技术集成研究与示范”(2018YFD0201100);甘肃省重点人才项目“党参根腐病综合防控技术集成、示范推广与人才培养”(2019RCXM072)。

摘　　要：为明确蒙古黄芪苗期立枯病害及其病原菌种类,采用组织分离法进行病原菌分离,并根据其培养性状、形态学和分子生物学rDNA-ITS特征进行鉴定。结果表明,该病害的病原菌为立枯丝核菌(Rhizotonia solani)。室内致病性测试结果表明,立枯丝核菌可引起蒙古黄芪苗期立枯病。本研究首次报道了蒙古黄芪苗期立枯病及其病原菌,可为生产上该病害的防治提供理论依据。In order to clarify the pathogen and pathogenicity of Rhizoctonia solani in Astragalus membranaceus var.mongholicus seedling stage,the pathogen was isolated by tissue isolation method and identified according to its culture traits,morphology and molecular biological characteristics.The results showed that the disease pathogens were identified as Rhizotonia solani.This study first reported Rhizoctonia solani and its pathogen in Astragalus membranaceus var.mongholicus seedling stage,which could provide a theoretical basis for the control of the disease in production.

关 键 词：苗期 蒙古黄芪 立枯病 病原鉴定 致病性测定 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]