HMGB1-RAGE信号通路和子痫前期发病机制相关性分析  

Correlation Analysis of HMGB1-RAGE Signaling Pathway and Pathogenesis of Preeclampsia

作　　者：王蕊[1] 魏莉 卢洪涛[1] 李冉冉[1] WANG Rui;WEI Li;LU Hongtao(Zaozhuang Maternal and Child Health Hospital, Shandong Zaozhuang 277100, China)

机构地区：[1]山东省枣庄市妇幼保健院医学遗传与产前筛查科,山东枣庄277100

出　　处：《河北医学》2021年第10期1612-1615,共4页Hebei Medicine

基　　金：山东省医药卫生科技发展计划项目,(编号:2017WSA04024)。

摘　　要：目的:探究高迁移率族蛋白B1(HMGB1)及其受体晚期糖基化终末产物(RAGE)信号通路和子痫前期发病机制相关性。方法:前瞻性选取2019年1月至2021年1月我院收治的64例子痫前期患者作为研究对象,根据疾病程度不同分为轻度组(n=34例)与重度组(n=30例),将同期我院30例健康孕妇作为对照组,检测三组血清及胎盘组织中HMGB1、RAGE、核转录因子-kp65(NF-kBp65)表达情况,对胎盘组织中HMGB1、RAGE、NF-kp65蛋白定位进行免疫组化试验,分析HMGB1、RAGE、NF-kBp65与子痫前期相关性。结果:重度组患者血清HMGB1、RAGE、NF-kBp65表达显著高于轻度组与对照组,差异有统计学意义(P<0.05);与对照组及轻度组相比,重度组胎盘组织中HMGB1、RAGE、NF-kBp65显著较高,差异有统计学意义(P<0.05);免疫组化试验显示重度组HMGB1、RAGE、NF-kBp65强阳性表达均高于轻度组及对照组(P<0.05),有统计学意义;子痫前期孕妇血清HMGB1、RAGE与NF-kBp65呈现出明显的正相关(r=0.387,P<0.05)。结论:HMGB1与RAGE在子痫前期患者血清及胎盘组织中呈现高表达,且与疾病严重程度有关,考虑HMGB1-RAGE活化kBp65信号通路是子痫前期重要发病机制,应予以重视。Objective:To investigate the correlation between high mobility group protein B1(HMGB1)and its receptor advanced glycation end product(RAGE)signaling pathway and the pathogenesis of preeclampsia.Methods:64 early epilepsy patients admitted between January 2019 and January 2021 were prospectively selected as the research objects and were divided into mild group(n=34 cases)and severe group(n=30),according to different degrees of disease.30 healthy pregnant women in the same period were selected as control group.Serum and placental tissue HMGB1,RAGE,nuclear transcription factors-kp65(NF-kBp65)expression,HMGB1 in placental tissue,RAGE,NF-kp65 protein localization of immunohistochemical test were tested in all groups.The correlation between HMGB1,RAGE,NF-KBP65 and preeclampsia was analyzed.Results:The expressions of HMGB1,RAGE and NF-KBP65 in serum of severe group were significantly higher than those of mild group and control group,with statistical significance(P<0.05).Compared with control group and mild group,HMGB1,RAGE and NF-KBP65 in placental tissue of severe group were significantly higher,with statistical significance(P<0.05).Immunohistochemical test showed that the strong positive expressions of HMGB1,RAGE and NF-KBP65 in severe group were higher than those in mild group and control group(P<0.05),which was statistically significant.Serum HMGB1 and RAGE were positively correlated with NF-KBP65 in pregnant women with preeclampsia(r=0.387,P<0.05).Conclusion:HMGB1 and RAGE are highly expressed in serum and placental tissues of patients with preeclampsia,and are related to the severity of the disease.Considering that HMGB1-RAGE activated KBP65 signaling pathway is an important pathogenesis of preeclampsia,attention should be paid to it.

关 键 词：高迁移率族蛋白B1 糖基化终末产物 子痫前期 核转录因子 

分 类 号：R71[医药卫生—妇产科学]