乙醇-正丙醇-磷酸二氢钠多元混合双水相体系萃取/检测湖水中的诺氟沙星残留  

Separation and Determination of Norfloxacin in Real Water Samples Using Binary Small Molecule Alcohol-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography

作　　者：郭东刚[1] 倪良 王良[1] GUO Dong-Gang;NI Liang;WANG Liang(Key Laboratory of Preparation and Application of Enwironmental Friendly Materials,Jilin Normal University,Ministry of Education,Changchun 130103,China;School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China)

机构地区：[1]吉林师范大学环境友好材料制备与应用教育部重点实验室,长春130103 [2]江苏大学化学化工学院,镇江212013

出　　处：《应用化学》2021年第7期847-856,共10页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(No.21407058)资助。

摘　　要：构建了乙醇-正丙醇-磷酸二氢钠(NaH_(2)PO_(4))多元混合双水相体系萃取/检测环境水样中的诺氟沙星,讨论了醇的用量、盐的种类及浓度、pH值、温度对诺氟沙星分配行为的影响,并采用响应曲面法(Response surface methodology,RSM)对实验条件进行优化。结果表明,在乙醇与正丙醇加入量为4 mL(体积比为1∶1),NaH_(2)PO_(4)的浓度为0.200 g/mL,温度为30℃,pH=5的最优条件下,诺氟沙星的萃取率为94.34%。该多元双水相体系较单元双水相体系相比,目标物的萃取率明显提升。A binary aqueous two-phase system(ATPS)based on ethanol-1-propanol-NaH_(2)PO_(4)system was constructed and applied for the separation and determination of norfloxacin(NOR)in real water samples.The influence factors on partition behaviors of NOR were discussed,including the types and the concentration of phase salts,the volume ratio of alcohols,the pH value and extraction temperature.The response surface methodology was used to determine the best experimental conditions for multi-factor experiments.The study shows that when the concentration of NaH_(2)PO_(4)is 0.200 g/mL,the volume of ethanol is 2 mL(the volume ratio of alcohols is 1∶1),pH value is 5,and the extraction temperature is 30℃,the extraction efficiency of NOR reaches 94.34%.Compared with the unit aqueous two-phase system,the extraction efficiency of the target compound by the multi-component aqueous two-phase system constructed in this paper is obviously improved.

关 键 词：多元混合双水相 小分子醇 萃取/检测 诺氟沙星 

分 类 号：O658.2[理学—分析化学]