用于细胞电穿孔的双极性脉冲发生器的研制  

Development of bipolar pulse generator for cell electroporation

作　　者：吴忠航[1] 石浩志 姜松 饶俊峰 WU Zhonghang;SHI Haozhi;JIANG Song;RAO Junfeng(Technology Innovation Platform of Zhoupu Hospital Affiliated to Shanghai Medical College,Shanghai 201318,China;School of Mechanical Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海健康医学院附属周浦医院科创平台,上海201318 [2]上海理工大学机械工程学院,上海200093

出　　处：《核技术》2021年第10期17-22,共6页Nuclear Techniques

基　　金：国家自然科学基金(No.51707122、No.12005128)资助。

摘　　要：脉冲电场下细胞电穿孔技术可应用于水处理、食品杀菌、组织消融等,而不同的电穿孔应用对脉冲的波形有不同的需求。本文提出了一种由一个特殊的全桥模块和多个半桥模块组成的新型脉冲发生器结构,除了能够产生双极性的方波脉冲电压之外,还可以通过仅改变开关时序实现阶梯波和窄脉冲与宽脉冲组合波。本文详细阐述了此新型电路的结构和工作原理,搭建了由12个开关构成的1个全桥模块和4个半桥模块组成的主电路;当每级电容充电220 V时,实现了±2 kV、脉宽1~10μs可调的双极性脉冲电压输出。此外,通过控制开关时序,可实现单极性9级阶梯电压以及宽窄脉冲组合波的输出。最后在马铃薯块茎处理实验中验证了该电源用于细胞电穿孔实验的可行性。[Background]Cell electroporation technology under pulsed electric field has been widely used in water treatment,food sterilization,tissue ablation and other applications.Different electroporation applications have different requirements for the pulse waveform.[Purpose]This study aims to propose a new pulse generator structure consisting of a special full bridge(FB)module and multiple half bridge(HB)modules to generate bipolar pulse voltages,stepped waves and combination of narrow and wide pulses can also be realized by only changing the switching sequence.[Methods]First of all,the structure and working principle of novel circuit were elaborated.Then,a main circuit structure composed of 1 full-bridge module and 4 half-bridge modules composed of 12 switches were designed and implemented.Bipolar pulse voltages,stepped waves and combination of narrow and wide pulses were realized by only changing the switching sequence.Finally,verification of the power supply for cell electroporation experiments was carried out in the potato tuber treatment experiment.[Results and Conclusions]When each level of capacitor is charged at 220 V,bipolar pulse voltage output with ±2 kV and adjustable pulse width of 1~10μs is realized.Furthermore,the output of a unipolar 9-level step voltage and a combination of wide and narrow pulses are achieved by controlling the switching sequence.Experiment of potato tuber treatment verified the feasibility of this pulse generator.

关 键 词：脉冲发生器 双极性 阶梯波 电穿孔 

分 类 号：TM832[电气工程—高电压与绝缘技术] TL8[核科学技术—核技术及应用]