不同剂量臭氧预处理污泥厌氧发酵产甲烷效果评价  

Evaluation of the Effect of Anaerobic Fermentation of Sludge Pretreated with Ozone in Different Dosages for Methane Production

作　　者：王太恒 陈彩霞 王旭东 李先国[1] 张大海[1] Wang Taiheng;Chen Caixia;Wang Xudong;Li Xianguo;Zhang Dahai(College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China)

机构地区：[1]中国海洋大学化学化工学院,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2021年第11期59-65,93,共8页Periodical of Ocean University of China

基　　金：山东省重点研究发展专项(ZR2020KB021);液化空气(中国)研发有限公司“生物制气”专项(20200216);中国海洋大学基本科研业务费项目(201762030)资助。

摘　　要：通过对二次沉淀池污泥浓缩后进行不同剂量的臭氧预处理,研究了污泥厌氧发酵过程中的产甲烷能力。实验证明,臭氧预处理能够促进污泥有机物的释放,微生物对有机物的利用率提高进而提升厌氧发酵产甲烷能力。浓缩污泥的SCOD含量及厌氧发酵中SCOD的去除率均随臭氧剂量增大而增加;甲烷产量与SCOD去除率呈正相关,当臭氧剂量在0.24 g/g TSS时污泥的产甲烷量最大,为空白组的1.88倍。微生物群落多样性分析发现,与产甲烷过程相关的菌群相对丰度均增大;厌氧发酵过程中主要的产甲烷菌为甲烷鬃毛菌(Methanosaeta),臭氧剂量的增加有利于产甲烷菌的生长,从而提升产甲烷效果。The sludge in the secondary sedimentation tank was concentrated and pretreated with different doses of ozone to study the methane production capacity of the sludge in the anaerobic fermentation process.Experiments have proved that ozone pretreatment can promote the release of sludge organic matter and the utilization of microorganisms to organic matter is improved,thereby enhancing the ability of anaerobic fermentation to produce methane.The SCOD content of concentrated sludge and the removal rate of SCOD in anaerobic fermentation increased with the increase of ozone dosage.The methane production is positively correlated with the SCOD removal rate.When the ozone dosage is 0.24 g/g TSS,the methane production from the sludge is the largest,which is 1.88 times that of the blank group.Analyzing the composition of microbial diversity,it was found that the relative abundance of bacteria associated with the process of methanogenesis increased.The main methanogen in the anaerobic fermentation process is Methanosaeta.The increase in ozone dose is conducive to the growth of methanogens,thereby enhancing the methanogenic effect.

关 键 词：臭氧预处理 厌氧发酵 甲烷 微生物群落 

分 类 号：S273.5[农业科学—农业水土工程]