负载PRP壳聚糖-明胶复合敷料止血和促愈合效果的初步评价  

The Hemostatic and Healing Effect of PRP Chitosan-gelatin Composite Dressing:A Preliminary Evaluation

作　　者：杨菁[1,2] 周谋[3] 林放 施琳颖 李艳辉 李文丹 单桂秋 YANG Jing;ZHOU Mou;LIN Fang(The First School of Clinical Medicine,Southern Medical University,Guangzhou 510515)

机构地区：[1]南方医科大学第一临床医学院,广州510515 [2]中山大学附属口腔医院检验科 [3]中国人民解放军南部战区总医院输血医学科

出　　处：《临床输血与检验》2021年第5期612-617,共6页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：军队后勤项目(No.BGZ 15C002)、(No.CWH17C019)资助。

摘　　要：目的通过建立兔肝脏出血模型,研究负载PRP壳聚糖-明胶复合敷料(CMC/GMs/PRP敷料)的止血和促愈合效果。方法将20只健康新西兰兔随机分4组,每组5只。分别为空白对照组(A组),实验组(B组)、阴性对照组(C组,创口敷普通纱布)、阳性对照组(D组,创口敷术益纱敷料)。建立兔肝脏出血模型后,记录每只兔的止血时间和出血量,并于术后第1、2、3、4周时进行主要血液学指标检测及组织学评价。结果CMC/GMs/PRP复合敷料的止血时间和出血量均少于其它三个对照组,其差异有统计学意义(P<0.05)。动态观察愈合过程中实验组白细胞变化最小,实验组肝损伤后的修复效果和安全性均优于其它组。结论负载PRP壳聚糖-明胶复合敷料具有良好的止血和促进创面愈合的作用,且有较高的安全性,具有进一步研究的价值和应用前景。Objective To study the hemostatic and effects of wound healing using chitosan-gelatin composite dressing loaded with platelet rich plasma(PRP)(CMC/GMs/PRP dressing)in a rabbit model of liver hemorrhage.Methods Twenty healthy New Zealand rabbits were randomly divided into 4 groups with 5 rabbits in each group:group A:control group,group B:CMC/GMs/PRP group,group C:negative control group(ordinary gauze),group D:positive control group(Muyisha gauze).The rabbit liver hemorrhage model was established.The hemostasis time and the amount of bleeding were recorded.The main hematological indicators and histological evaluations were performed at the first 1,2,3,4 weeks after the operation.Results The hemostasis time and bleeding volume in the CMC/GMs/PRP group were less than those in other three control groups(P<0.05).During wound healing process,changes in white blood cell counts in the CMC/GMs/PRP group were minimal and repair of liver injury and safety in the CMC/GMs/PRP group were both better than those in other groups.Conclusion The PRP chitosan-gelatin composite dressing is capable of hemostasis and promoting wound healing,and also is safety.It has further research value and application prospects.

关 键 词：富血小板血浆 壳聚糖 明胶 止血 愈合 

分 类 号：R318[医药卫生—生物医学工程]