艾地苯醌对血小板生物学特性影响的初步研究  

A Preliminary Study on the Effect of Idebenone on the Biological Characteristics of Platelets

作　　者：林俊填[1] 麦志周 杨富燕 伍伟健[1] 刘运芝[1] 余玉婷[1] LIN Jun-tian;MAI Zhi-zhou;YANG Fu-yan(Foshan Central Blood Station,528000)

机构地区：[1]广东省佛山市中心血站,528000 [2]佛山市第一人民医院禅城医院 [3]佛山市南海区人民医院

出　　处：《临床输血与检验》2021年第5期628-631,共4页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：广东省佛山市自筹经费类科技计划项目(No.1920001000780)资助。

摘　　要：目的本研究拟通过艾地苯醌对血小板生物学特性的影响,以期探讨其在血小板保存方面的应用价值。方法体外实验中,富小板血浆加入终浓度为10μg/mL艾地苯醌作为实验组,检测血小板粘附和聚集功能,血浆丙二醛、还原型谷胱甘肽、以及血小板膜表面CD62p和线粒体膜电位的变化。结果与对照组相比,实验组血小板的粘附率和聚集率均有一定程度的下降(P<0.05);血浆丙二醛水平降低(P<0.05)而谷胱甘肽水平升高(P<0.05);CD62p的阳性率无显著性变化,但JC-1的阳性率有显著性增加(P<0.05)。结论10μg/mL艾地苯醌有利于血小板线粒体的代谢,但是对血小板的粘附和聚集功能产生一定的抑制作用。Objective To explore the application value of idebenone in platelet preservation through its effect of on platelet biological characteristics.Methods In vitro experiment,platelet-rich plasma was added idebenone with the final concentration of 10μg/mL as the experimental group.We detected the platelet adhesion and aggregation function,plasma malondialdehyde(MDA)concentration,reduced glutathione(GSH)concentration,and CD62p expression on platelet membrane surface and JC-1 on mitochondrion surface.Results Compared with the control group,platelet adhesion and aggregation rate decreased to a certain extent(P<0.05);The MDA level decreased(P<0.05)while reduced GSH level increased(P<0.05);The positive rate of CD62p showed no significant change,but the positive rate of JC-1 increased significantly(P<0.05).Conclusion Idebenone would be beneficial to the metabolism of platelet mitochondria,but it has a certain inhibitory effect on platelet adhesion and aggregation with the concentration of 10μg/mL.

关 键 词：艾地苯醌 血小板 粘附 聚集 CD62P 

分 类 号：R331.1[医药卫生—人体生理学]