脂质体的特异性识别与单颗粒电化学检测  

Specific Molecular Recognition of Liposome Nanoparticles and Single-particle Electrochemical Detection

作　　者：王克青 李自若 费进波[1,2] 王晨蕾 崔巍 赵洁[1] 李峻柏[1,2] Ke-qing Wang;Zi-ruo Li;Jin-bo Fei;Chen-lei Wang;Wei Cui;Jie Zhao;Jun-bai Li(CAS Key Laboratory of Colloid,Interface and Chemical Thermodynamics,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190;University of Chinese Academy of Sciences,Beijing 100049)

机构地区：[1]中国科学院化学研究所胶体、界面与化学热力学重点实验室,北京100190 [2]中国科学院大学,北京100049

出　　处：《高分子学报》2021年第8期1024-1031,I0005,共9页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号21961142022,21872150和22072160)资助项目。

摘　　要：脂质体载药系统已在许多疾病的治疗中发挥重要作用.体外构建并模拟脂质体与生物膜特异性识别相互作用,对于探索脂质体在生物体内的稳定性,开发新型高效和智能化药物递送系统具有重要的现实意义.本文通过分子组装技术构筑了稳定的磷脂囊泡-脂质体,利用微加工技术制备金微电极,然后在其表面进行亲合素修饰,借助电化学工作站检测脂质体单颗粒的碰撞行为,以此建立一种检测脂质体在体外和生物体内存在的技术平台.实验证明,在脂质体中引入生物素分子,通过与微电极表面的亲合素分子产生特异性识别,能显著增加单颗粒碰撞的频率和强度.这些定量的数据为研究脂质体与生物膜动态互作提供了十分有效和准确的检测手段,为验证脂质体载药体系在生物内的稳定性和释放速率建立了新方法.Liposomes delivery drug systems show obvious advantages in the treatment of many diseases.Constructing new types of liposome based drugs is of great importance to develop more efficient novel drug delivery systems.Herein,the present work demonstrated that Au microelectrode was chemically modified with avidin through Au-S bond(Avidin-S-Au microelectrode)and liposomes modified with the biotin(Biotinliposome)were constructed through molecular assembly.Furthermore,an electrochemical detection device was constructed to recognize liposomes through single nanoparticle collision.Compared with pure liposomes,biotin modified liposome displayed a higher collision frequency and intensity with Avidin-S-Au microelectrode.Importantly,with the increasing concentration of biotin-liposome,the collision frequency increases as well.Taking the single liposome collision to the gold microelectrode into account,one can obtain quantitative information of the liposome size and concentration through the frequency and intensity of single nanoparticle collision.These findings represent an unusual case to achieve high sensitivity detection of individual liposomes with poor electrochemical activity and at very low concentration.The present work creates a simple and effective way to detect the stability of liposomes and their interaction with biomembranes.

关 键 词：脂质体 超分子组装 单颗粒碰撞 电化学检测 分子识别 

分 类 号：O657.1[理学—分析化学] TQ460.4[理学—化学]