2017-2019年四川部分地区PRRSV ORF5基因的遗传演化分析  

Genetic Evolution Analysis of Porcine Reproductive and Respiratory Syndrome Virus ORF5 Gene in Some Regions of Sichuan Province during 2017 to 2019

作　　者：唐伟明 秦立廷 董友富 陈婷 罗刚 卞婷 张振东 TANG Wei-ming;QIN Li-ting;DONG You-fu;CHEN Ting;LUO Gang;BIAN Ting;ZHANG Zhen-dong(College of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang 212018,China;Shandong New Hope Liuhe Group Co.,Ltd,Qingdao 266000,China;Qingdao Jiazhi Biotechnology Co.,Ltd,Qingdao 266000,China)

机构地区：[1]江苏科技大学生物技术学院,江苏镇江212018 [2]山东新希望六和集团有限公司,山东青岛266000 [3]青岛嘉智生物技术有限公司,山东青岛266000

出　　处：《中国兽医杂志》2021年第5期5-9,共5页Chinese Journal of Veterinary Medicine

基　　金：江苏省自然科学基金青年基金(BK20201005)。

摘　　要：为了解2017—2019年四川地区猪繁殖与呼吸综合征病毒(PRRSV)的流行情况,对收集的47份PRRSV阳性样品进行ORF5基因的扩增、测序,应用DNASTAR和MEGA软件对核苷酸序列及其推导的氨基酸序列进行同源性和遗传演化分析。结果显示,47条ORF5序列之间的核苷酸同源性为84.2%~100.0%,与PRRSV原始毒株LV和VR-2332的核苷酸同源性分别为60.7%~64.6%、84.9%~99.3%,表明分离毒株均属于PRRSV2。对其中35个ORF5基因序列进行遗传演化分析,结果发现,四川地区PRRSV的流行主要以谱系1、5和8为主,各占比34.3%(12/35)、37.1%(13/35)和28.6%(10/35)。此外,氨基酸分析显示,11个分离株的ORF5基因在第34位存在1个氨基酸的缺失,占比31.4%(11/35),特别是该11个缺失分离株均属于谱系1,表明该缺失可能存在毒株特异性。本试验丰富了四川地区PRRSV的流行病学数据,为该地区猪繁殖与呼吸综合征的防控提供了科学依据。To study the prevalence and evolution of PRRSV in Sichuan province from 2017 to 2019,47 PRRSV RT-PCR positive samples were used to amplify and sequence of ORF5 genes.The homology of the sequenced nucleotides and deduced amino acids was analyzed by DNASTAR and MEGA softwares.The results showed that 47 ORF5 genes shared 84.2%-100.0%,60.7%-64.6%and 84.9%-99.3%nucleotide homology with each other,PRRSV prototype strain LV and VR-2332,respectively,indicating that all the isolates belong to PRRSV2.The phylogenetic tree based on 35 and referenced ORF5 genes suggested the lineage 1,5,8 were predominant strains in Sichuan with 34.3%(12/35),37.1%(13/35)and 28.6%(10/35)proportion,respectively.Besides,the amino acid analysis showed that 1aa deletion at site 34 in 11 isolates,with a 31.4%(11/35)proportion.Interestingly,all the 11 isolates belonged to lineage 1,suggesting that the deletion may be strain specific.Taken together,our study enriches the data of PRRSV molecular epidemiology and will provide scientific basis for controlling PRRS in Sichuan.

关 键 词：猪繁殖与呼吸综合征病毒 ORF5基因 遗传演化 缺失 

分 类 号：S852.651[农业科学—基础兽医学]