复方灵芝对小鼠镇静催眠作用的研究及机制初探  

The Study and Mechanism Analysis of the Sedative and Hypnotic Effects of Compound of Ganoderma lucidum in Mice

作　　者：陈春玲 梁大红 田佳 陈文双 张新乐 许碧莲 许卫铭 吴铁 陈光华 CHEN Chun-ling;LIANG Da-hong;TIAN Jia;CHEN Wen-shuang;ZHANG Xin-le;XU Bi-lian;XU Wei-ming;WU Tie;CHEN Guang-hua(College of Medicine,Guangdong Medical University,Zhanjiang 524023,China;Affiliated Hospital of Guangdong Medical University,Zhanjiang 524023,China)

机构地区：[1]广东医科大学药学院,广东湛江524023 [2]广东医科大学附属医院,广东湛江524023

出　　处：《中国兽医杂志》2021年第5期74-78,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(81373499);广东省医学科学技术研究基金(A2018417)。

摘　　要：为考察复方灵芝对小鼠的镇静、催眠作用,并研究其对脑内神经递质含量的影响。将小鼠随机分为空白对照组[蒸馏水,0.2 mL/(10 g·bw)]、阳性药物组[艾司唑仑,0.26 mg/(10 g·bw)]、复方灵芝组[126 mg/(10 g·bw),以生药量计],每组10只,小鼠每天灌胃给药1次,连续给药8 d。以小鼠入睡率、睡眠潜伏期和睡眠持续时间为药效指标,考察戊巴比妥钠协同下复方灵芝的镇静、催眠作用。ELISA测定小鼠脑匀浆液中γ-氨基丁酸(GABA)、谷氨酸(Glu)、谷氨酰胺(Gln)、5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)含量。结果显示,与空白对照组比较,阈下剂量的戊巴比妥钠可使复方灵芝组小鼠入睡率极显著增加(P<0.01),阈上剂量的戊巴比妥钠可使复方灵芝组小鼠睡眠潜伏期极明显缩短(P<0.01)。与空白对照组比较,复方灵芝组小鼠脑内GABA含量极显著上升(P<0.01),Glu、Gln含量极显著下降(P<0.01)。结果表明复方灵芝可能通过增加小鼠脑内GABA含量,降低Glu、Gln含量,起到镇静、催眠的作用。To study the sedative and hypnotic effects of compound of Ganoderma lucidum on mice and the neurotransmitter contents in the brain,mice were randomly divided into blank control group[water,0.2mL/(10 g·bw)],positive drug group[estazolam,0.26mg/(10 g·bw)],and compound of Ganoderma lucidum group[126 mg/(10 g·bw),calculated by crude drug],with 10 mice in each group.Each group was given the relevant medicine intragastrically once a day,for consecutive 8 days.The mice were then given subthreshold doses and threshold doses of pentobarbital sodium to induce sleep,and their sleep status,sleep latency,and sleep duration were recorded.The contents ofγ-aminobutyric acid(GABA),glutamate(Glu),glutamine(Gln),5-hydroxytryptamine(5-HT)and 5-hydroxyindoleacetic acid(5-HIAA)in brain homogenate were determined by enzyme-linked immunosorbent assay.The results showed that compared with the blank control group,compound of Ganoderma lucidum group had an increased sleep rate and the duration of sleep,shortened incubation period of sleep after given subthreshold dose pentobarbital sodium(P<0.01),and an shorten sleep latency after given suprathreshold dose pentobarbital sodium.Compared with the blank control group,brain GABA levels in comprund of Ganoderma lucidum group significantly increased(P<0.01),while the contents of Glu and Gln remarkably decreased(P<0.01).The results indicate that compound of Ganoderma lucidum may play a calming and hypnotic role by increasing the content of GABA in the brain of mice and reducing the content of Glu and Gln.

关 键 词：复方灵芝 镇静 催眠 戊巴比妥钠 小鼠 

分 类 号：S567.31[农业科学—中草药栽培]