钩吻素己对NMDA受体的抑制作用  

The Inhibition Effect of Gelsenicine on NMDA Receptor

作　　者：彭凌峰 杨杰 孙志良[1,2,3] PENG Ling-feng;YANG Jie;SUN Zhi-liang(College of Veterinary Medicine,Hunan Agriculture Unversity,Changsha 410125,China;Hunan Engineering Research Centre of Veterinary Drug,Changsha 410125,China;Hunan Collaborative Innovation for Ultilization of Botanical Function Ingredients,Changsha 410125,China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410125 [2]湖南省兽药工程技术研究中心,湖南长沙410125 [3]湖南省植物功能成分利用协同创新中心,湖南长沙410125

出　　处：《中国兽医杂志》2021年第5期79-84,I0009,共7页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划(2017YFD0501403)。

摘　　要：为了揭示钩吻素己对N-甲基-D-天冬氨酸(NMDA)受体活性的抑制作用,将钩吻素己、喹啉酸、钩吻素己+喹啉酸、地佐环平+喹啉酸分别作用于小鼠神经母瘤细胞(Neuro-2a),运用噻唑蓝(MTT)试验方法检测细胞活力度;采用5-乙炔基-2′-脱氧胞苷(EdU)细胞增殖试验方法检测细胞增殖;应用Western Blot试验方法研究钩吻素己对NMDA受体的抑制作用效果。结果显示,钩吻素己拮抗喹啉酸对Neuro-2a的细胞毒性,提高细胞的增殖能力(P<0.05),并且钩吻素己通过调控N-甲基-D-天冬氨酸ε2(NMDAε2)蛋白降低N-甲基-D-天冬氨酸ζ1(NMDAζ1)蛋白活性(P<0.05)。本试验结果表明钩吻素己能够有效抑制NMDA受体蛋白活性。In order to investigate the inhibitory effect of gelsenicine on N-methyl-D-aspartic acid(NMDA)receptor activity,Neuro-2a cells were treated with gelsenicine,quinolinic acid,gelsenicine plus quinolinic acid,and dizocilpine plus quinolinic acid respectively.And then,cell viabilities were measured by MTT assay.The cell proliferation was measured by 5-ethynyl-2′-deoxyuridine(EdU)assay.Western Blot assay was used to verify the effect of gelsenicine on NMDA receptor.The results showed that the gelsenicine reduced the toxicity of quinolinic acid and increased the ability of cell proliferation(P<0.05).Furthermore,gelsenicine reduced N-methyl-D-aspartic acidζ1(NMDAζ1)protein activity by regulating N-methyl-D-aspartic acidε2(NMDAε2)protein avtivity(P<0.05).These data suggest that gelsenicine can inhibit NMDA receptor activity.

关 键 词：钩吻素己 喹啉酸 地佐环平 NMDA受体 

分 类 号：R285.5[医药卫生—中药学]