LINC00612靶向结合Bcl-2抑制Aβ1-42孵育的神经元凋亡  

LINC00612 targeted Bcl-2 to inhibit the apoptosis of neurons incubated with Aβ1-42

作　　者：彭乾 赵德丰 张健[1] 薛一雪[3] 姜季委 商秀丽[1] PENG Qian;ZHAO Defeng;ZHANG Jian(Department of Neurology,the First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [2]中国医科大学,辽宁沈阳110001 [3]中国医科大学神经生物学教研室,辽宁沈阳110001

出　　处：《中风与神经疾病杂志》2021年第9期810-812,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨LINC00612对β-淀粉样蛋白(amyloid proteinβ,Aβ)1-42孵育的SH-SY5Y细胞凋亡的影响。方法利用Aβ1-42孵育的SH-SY5Y细胞建立阿尔茨海默病神经元损伤模型。应用qRT-PCR实验检测Aβ1-42孵育的SH-SY5Y细胞中LINC00612的表达。将LINC00612过表达质粒转染至Aβ1-42孵育的SH-SY5Y细胞,Western blot实验检测Bcl-2的表达,Annexin V-FITC/PI染色法检测细胞凋亡。RNA-pulldown和RIP实验检测LINC00612与Bcl-2的结合作用。结果LINC00612在Aβ1-42孵育的SH-SY5Y细胞中低表达。过表达LINC00612显著增加Bcl-2表达量,降低细胞凋亡。RNA-pulldown和RIP实验结果显示LINC00612能够与直接Bcl-2结合。结论过表达LINC00612能够上调Bcl-2表达量,抑制Aβ1-42孵育的SH-SY5Y细胞凋亡。Objective To investigate the effect of LINC00612 on apoptosis of SH-SY5Y cells incubated with Aβ1-42.Methods Neuronal injury model of Alzheimer’s disease were established by SH-SY5Y cells incubated with Aβ1-42.The expression of LINC00612 in SH-SY5Y cells incubated with Aβ1-42 was detected by qRT-PCR.The LINC00612 overexpressed plasmid was transfected into SH-SY5Y cells incubated with Aβ1-42,and the expression of Bcl-2 was detected by Western blot.Apoptosis was detected by Annexin V-FITC/PI staining.RNA-pulldown and RIP experiments were performed to detect the binding effect of LINC00612 and Bcl-2.Results LINC00612 was low expressed in SH-SY5Y cells incubated with Aβ1-42.Overexpression of LINC00612 significantly increased Bcl-2 expression and decreased apoptosis.RNA-pulldown and RIP results showed that LINC00612 could bind to Bcl-2.Conclusion Overexpression of LINC00612 can up-regulate Bcl-2 expression and inhibit the apoptosis of SH-SY5Y cells incubated with Aβ1-42.

关 键 词：LINC00612 BCL-2 β-淀粉样蛋白1-42 SH-SY5Y细胞 

分 类 号：Q25[生物学—细胞生物学]