枳实通降颗粒对术后肠梗阻大鼠胃肠动力和白细胞介素6、肿瘤坏死因子α的影响  

Effect of Zhishi Tongjiang Granule on Gastrointestinal Motility,Interleukin-6 and Tumor Necrosis Factor-α in Rats with Postoperative Intestinal Obstruction

作　　者：李倩 莫黎[2] 何永恒 LI Qian;MO LI;HE Yongheng(the Second Clinical College of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Hunan Changsha 410007,China;Dept.of Proctology,the Second Affiliated Hospital of Hunan University of Chinese Medicine,Hunan Changsha 410005,China;Dept.of Proctology,Affiliated Hospital of Hunan Academy of Chinese Medicine,Hunan Changsha 410006,China)

机构地区：[1]湖南中医药大学第二中医临床学院,湖南长沙410007 [2]湖南中医药大学第二附属医院肛肠五科,湖南长沙410005 [3]湖南省中医药研究院附属医院肛肠科,湖南长沙410006

出　　处：《中国医院用药评价与分析》2021年第9期1025-1027,1031,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：2021年度湖南省中医药科研计划项目(No.2021100)。

摘　　要：目的:观察枳实通降颗粒对术后肠梗阻大鼠胃肠动力和血清白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)表达的影响,探讨枳实通降颗粒对术后胃肠功能的恢复作用及机制。方法:选取SD大鼠30只,雌雄各半,随机分为空白组、模型组及实验组,每组10只。模型组、实验组大鼠开腹,建立术后肠梗阻模型;术后24 h,测量胃肠推进率、胃残留物质量,并取靠近幽门部小肠2 cm行苏木精-伊红染色制图,观察肠黏膜损伤情况。结果:与空白组相比,实验组、模型组大鼠胃肠推进率显著降低,胃内容物质量和血清IL-6、TNF-α含量显著升高,差异均有统计学意义(P<0.01);实验组大鼠胃肠推进率较模型组有明显升高,胃内容物质量和血清IL-6、TNF-α含量显著降低,差异均有统计学意义(P<0.01);病理图片结果显示,模型组大鼠的小肠黏膜炎症细胞渗出及黏膜坏死较空白组更明显,实验组大鼠的炎症细胞渗出情况较模型组缓解,黏膜坏死少见。结论:枳实通降颗粒能改善术后肠梗阻大鼠胃肠动力,抑制血清IL-6、TNF-α表达升高,促进术后胃肠功能恢复。OBJECTIVE: To observe the effect of Zhishi Tongjiang granule on gastrointestinal motility,serum interleukin-6( IL-6) and tumor necrosis factor-α( TNF-α) in rats with postoperative intestinal obstruction,so as to explore the restorative effect and mechanism of Zhishi Tongjiang granule on postoperative gastrointestinal function.METHODS: Totally 30 SD rats were selected,with half male and half female,which were randomly divided into blank group,model group and experimental group,with 10 rats in each group. The rats in model group and experimental group were given laparoscopy to establish postoperative intestinal obstruction model;at 24 h after surgery,the gastrointestinal propulsion rate and gastric residue mass were measured,and 2 cm close to the small intestine pylorus was taken for Hematoxylin-Eosin staining to observe the intestinal mucosal damage. RESULTS: Compared with the blank group,the gastrointestinal propulsion rates in experimental group and model group decreased significantly,the gastric residue mass,serum IL-6 and TNF-α contents increased significantly,with statistically significant differences( P<0. 01);the gastrointestinal propulsion rate of experimental group was significantly higher than that of the model group,the gastric residue mass,serum IL-6 and TNF-α contents of experimental group were significantly lower than those of the model group,with statistically significant differences( P<0. 01). Results of pathological images indicated that the inflammatory exudation and necrosis of small intestinal mucosa in the model group were more obvious than that of the blank group,the inflammatory exudation was alleviated and mucosal necrosis was less in the experimental group than compared with the model group. CONCLUSIONS: Zhishi Tongjiang granule can improve the gastrointestinal dynamics of rats with postoperative intestinal obstruction,inhibit the elevation of serum IL-6 and TNF-α,and promote the recovery of postoperative gastrointestinal function.

关 键 词：枳实通降颗粒 术后肠梗阻 胃肠动力 白细胞介素6 肿瘤坏死因子Α 

分 类 号：R932[医药卫生—生药学]