白背三七提取物联合葛根素对2型糖尿病大鼠的降糖作用  

Hypoglycemic effect of divaricate gynura extract combined with puerarin on type 2 diabetic rats

作　　者：王海霞[1] 王旭艳 李晓霞[1] Wang Haixia;Wang Xuyan;Li Xiaoxia(Department of Pharmacy,the Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030053,China;Department of Clinical Medicine,Shanxi University of Traditional Chinese Medicine,Taiyuan 030025,China)

机构地区：[1]山西医科大学附属第二医院药剂科,太原030053 [2]山西省中医药大学临床医学系,太原030025

出　　处：《中华实验外科杂志》2021年第10期1956-1959,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨白背三七提取物联合葛根素对2型糖尿病大鼠的降糖作用及其机制。方法采用腹腔注射链脲佐菌素诱导2型糖尿病大鼠模型;将造模成功大鼠通过简单随机化方法分为三七黄酮联合葛根素组(80 mg/kg+80 mg/kg),三七黄酮组(80 mg/kg),葛根素组(80 mg/kg),西格列汀组(2 mg/kg),模型对照组,同时设有正常对照组,每组各10只;观察各给药组对糖尿病模型大鼠空腹血糖(FBG)、糖化血红蛋白(GHB)、超氧化物歧化酶(superoxide dismutase,SOD)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的影响。计量资料应用t检验、方差分析、Spearman相关分析。结果通过建立2型糖尿病模型大鼠,检测各给药方法对模型大鼠的FBG、GHB、SOD、TNF-α的影响,通过实验验证,证实三七黄酮联合葛根素组的FBG值低于模型对照组[(12.03±5.07) mmol/L比(27.04±5.46) mmol/L],差异具有统计学意义(t=8.434,P<0.01);三七黄酮联合葛根素组的GHB值低于模型对照组[(13.77±5.89) ng/ml比(24.68±4.77) ng/ml],差异具有统计学意义(t=8.324,P<0.01);三七黄酮联合葛根素组SOD值高于模型对照组[(253.32±24.43) U/ml比(218.98±21.38) U/ml],差异具有统计学意义(t=7.991,P<0.05);三七黄酮联合葛根素组指标低于模型对照组[(456.32±51.43) pg/g比(619.42±54.71) pg/g],差异具有统计学意义(t=8.677,P<0.05)。而三七黄酮组、葛根素组与模型对照组比较,在FBG、GHB、SOD、TNF-α上无统计学差异。结论三七黄酮联合葛根素能够改善糖尿病大鼠高血糖症状,其机制可能与其改善脂质代谢和抗脂质过氧化有关。Objective Research the mechanism of hypoglycemic effect of divaricate gynura extract combined with puerarin on type 2 diabetic rats.Methods Type 2 diabetes rats were induced by intraperitoneal injection of STZ.The successfully modeled rats were divided into notoginseng flavone combined with puerarin group(80 mg/kg+80 mg/kg),notoginseng flavone group(80 mg/kg),puerarin group(80 mg/kg),sitagliptin(2 mg/kg),model control group,and normal control group,each with 10 rats;observe the effects of each administration group on fasting blood glucose(FBG),glycosylated hemoglobin(GHB),superoxide dismutase(SOD),tumor necrosis factor-α(TNF-α),Measurement data were analyzed by t test,analysis of variance,and Spearman correlation analysis,expressed as mean±standard deviation(Mean±SD),and P<0.05 was considered statistically significant.Results Through the establishment of type 2 diabetes model rats,the effects of various administration methods on FBG,GHB,SOD,and TNF-αof the model rats were tested.The results showed that the FBG value of the notoginseng flavone combined with puerarin group was lower than the model control group[(12.03±5.07)mmol/L vs.(27.04±5.46)mmol/L],the difference was statistically significant(t=8.434,P<0.01);the GHB value of the notoginseng flavone combined with puerarin group was lower than the model control group[(13.77±5.89)ng/ml vs.(24.68±4.77)ng/ml],the difference was statistically significant(t=8.324,P<0.01);the SOD value of the notoginseng flavone combined with puerarin group was higher than the model control group[(253.32±24.43)U/ml vs.(218.98±21.38)U/ml],the difference was statistically significant(t=7.991,P<0.05);the TNF-αvalue of the notoginseng flavone combined with puerarin group was lower than the model control group[(456.32±51.43)pg/g vs.(619.42±54.71)pg/g],the difference was statistically significant(t=8.677,P<0.05).However,there was no statistical difference in FBG,GHB,SOD,TNF-αbetween the notoginseng flavone group,puerarin group and the model control group.Conclusion Panax notog

关 键 词：白背三七 葛根素 炎症 氧化应激 2型糖尿病 

分 类 号：R285.5[医药卫生—中药学]