水稻温敏型淡绿叶突变体tsv1的鉴定及遗传分析  

Characterization and Genetic Analysis of a Temperature-sensitive Virescent Mutant tsv1 in Rice

作　　者：朱宏素 张杰[1] 黎腊梅 唐诗闻 余德金 田敏 郭发平 陈永军[1] 胡运高[1] 彭友林[1] ZHU Hong-su;ZHANG Jie;LI La-mei;TANG Shi-wen;YU De-jin;TIAN Min;GUO Fa-ping;CHEN Yong-jun;HUYun-gao;PENG You-lin(Rice Research Institute,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China)

机构地区：[1]西南科技大学水稻研究所,四川绵阳621010

出　　处：《杂交水稻》2021年第5期58-64,共7页Hybrid Rice

基　　金：国家重点研发计划(2017YFD0100201);国家自然科学基金(32001491);四川省教育厅重点项目(18ZA0507);水稻生物学国家重点实验室开放课题(160202)。

摘　　要：以武运粳21为材料经EMS诱变筛选获得一个稳定的温敏型淡绿叶突变体tsv1。与野生型相比,tsv1苗期叶色较淡,苗期和分蘖期叶片的叶绿素含量极显著降低,净光合速率降低,产量构成因素和农艺性状受到严重不良影响。在20℃处理下,tsv1光合色素含量极低,幼苗完全黄化,植株矮小;在28℃处理下,tsv1幼苗叶片光合色素含量虽较野生型显著降低,但明显高于20℃处理,叶片呈淡绿色;而32℃处理下的tsv1幼苗叶片表型与野生型趋于一致,色素含量略高于28℃处理。将20℃处理后的幼苗转移至28℃继续处理1周后,tsv1叶色由黄化转变为淡绿色,色素含量上升,株高变高。上述结果表明tsv1表型受温度影响。遗传分析表明,温敏型淡绿叶突变性状受1对隐性核基因控制。研究结果为该突变体进一步的基因克隆和功能探索奠定了基础。A temperature-sensitive virescent mutant(tsv1) with genetically stable mutational trait was obtained from the progeny of a japonica rice variety Wuyunjing 21 mutagenized by ethyl methane sulfonate(EMS). Compared with the wild type, the mutant tsv1 had virescent leaf starting from the seedling stage, significantly decreased chlorophyll content at the seedling stage and tillering stage, resulting in a serious adverse effect on the net photosynthetic rate, yield components and agronomic traits. Under the condition of 20 ℃, tsv1 displayed the seedling in yellow with short plant and extremely low photosynthetic pigment content;under the condition of 28 ℃, tsv1 showed virescent leaf with significantly lower photosynthetic pigment content than that of the wild type but remarkably higher than that of the treatment of 20 ℃;but under the condition of 32 ℃, tsv1 showed a similar phenotype to the wild type with a lightly higher pigment content than that of the treatment of 28 ℃. After the seedlings treated at 20 ℃ were transferred to 28 ℃ for a week, tsv1 turned to virescent from yellow, and the pigment content and plant height increased. The above results indicated that the phenotype of tsv1 was affected by temperature. Genetic analysis showed that the mutational phenotype of temperature-sensitive virescent leaf was controlled by a single recessive nuclear gene. The results laid a foundation for further gene cloning and functional exploration of the mutant.

关 键 词：水稻 淡绿叶 温敏 突变体 tsv1 遗传 

分 类 号：S511.01[农业科学—作物学] S311