松萝酸通过PI3K/Akt通路对大鼠皮肤创面愈合及瘢痕增生的作用  

Effects of Usnic Acid on Skin Wound Healing and Scar Hyperplasia in Rats Through PI3K/Akt Pathway

作　　者：王晓妮[1] 关立锋[1] 罗启云 WANG Xiaoni;GUAN Lifeng;LUO Qiyun(Department of Burn Plastic Surgery,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院烧伤整形美容科,宁夏银川750004

出　　处：《中国皮肤性病学杂志》2021年第10期1111-1118,1131,共9页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金项目(81760558)。

摘　　要：目的基于磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(Akt)通路探讨松萝酸对大鼠皮肤创面愈合及瘢痕增生的作用。方法70只SD雄性大鼠取15只为正常组,另48只背部皮肤创伤模型大鼠随机分为模型组、阳性药物组、松萝酸组,各16只。观察大鼠不同时间皮肤创面愈合率,记录创面完全愈合时间、瘢痕厚度;酶联免疫吸附(ELISA)法检测创面组织白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;免疫组织化学法检测血管内皮细胞生长因子(VEGF)、ɑ平滑肌肌动蛋白(αSMA)累积光密度;苏木精-伊红(HE)染色法观察创面组织病理学形态;蛋白质印迹法检测磷酸化PI3K(p-PI3K)、磷酸化Akt(p-Akt)、磷酸化-哺乳动物雷帕霉素靶蛋白(p-mTOR)蛋白相对表达量。结果与模型组比较,阳性药物组、松萝酸组各时段创面愈合率升高,创面完全愈合时间缩短,瘢痕厚度降低(P<0.05);与正常组比较,模型组、阳性药物组、松萝酸组各时段IL-6、TNF-α水平升高,而阳性药物组、松萝酸组低于模型组(P<0.05);与模型组比较,阳性药物组、松萝酸组各时段VEGF、αSMA累积光密度升高(P<0.05);与正常组比较,模型组、阳性药物组、松萝酸组不同时段p-PI3K、p-Akt、p-mTOR蛋白相对表达量降低,模型组低于阳性药物组、松萝酸组(P<0.05)。结论松萝酸可促进皮肤创伤大鼠创面愈合,控制创面瘢痕过度增生,作用机制可能与调节PI3K/Akt通路有关。Objective To investigate the effects of usnic acid on skin wound healing and scar hyperplasia in rats based on phosphatidylinositol-3 kinase(PI3K)/protein kinase B(Akt)pathway.Methods Fifteen of 70 male SD rats were selected as normal group,and the other 48 rats with back skin trauma were randomly divided into model group,positive drug group,and usnic acid group,each with 16 rats.The healing rate of skin wound was observed at different time,and the wound healing time and scar thickness were recorded.The levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA).Immunohistochemical was used to detect the cumulative optical density of vascular endothelial growth factor(VEGF)and alpha-smooth muscle actin(αSMA).Hematoxylin eosin(HE)staining was used to observe the pathological morphology of the wound.The relative expression of phosphorylate-PI3K(p-PI3k),phosphorylate-Akt(p-Akt)and phosphorylate-mammalian target of rapamycin(p-mTOR)were detected by Western blot.Results Compared with the model group,the wound healing rate in the positive drug group and the usnic acid group was increased,the wound healing time was shortened,and the scar thickness was decreased(P<0.05).Compared with the normal group,the levels of IL-6,TNF-αin the model group,positive drug group and usnic acid group were increased,and the positive drug group and usnic acid group were lower than the model group(P<0.05).Compared with the model group,the cumulative optical density of VEGF andαSMA increased in positive drug group and usnic acid group at different time points(P<0.05).Compared with the normal group,the relative expression of p-PI3k,p-Akt and p-mTOR protein in the model group,positive drug group and usnic acid group was lower than that in the positive drug group and usnic acid group(P<0.05).Conclusion Usnic acid can promote wound healing in rats with skin trauma and control the hyperplasia of wound scars.The mechanism of action may be related to the regulation of PI3K/Akt pathway

关 键 词：皮肤创伤 创面愈合 瘢痕增生 松萝酸 磷脂酰肌醇-3激酶 蛋白激酶B 

分 类 号：R285.5[医药卫生—中药学] R739.5[医药卫生—中医学]