Characterizing in situ poroelastic properties of cytoplasm by the translation of a rigid spherical inclusion  

作　　者：Xuechao Sun Xin Chen Ming Wang Moxiao Li Yonggang Liu Shaobao Liu Tian Jian Lu 

机构地区：[1]State Key Laboratory of Mechanics and Control of Mechanical Structures,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China [2]Xi'an Modern Chemistry Research Institute,Xi'an 710065,China [3]State Key Laboratory for Strength and Vibration of Mechanical Structures,School of Aerospace,Xi'an Jiaotong University,Xi'an 710049,China [4]Bioinspired Engineering&Biomechanics Center(BEBC),Xi'an Jiaotong University,Xi'an 710049,China [5]Key Laboratory of Biomedical Information Engineering of Ministry of Education,School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049,China

出　　处：《Acta Mechanica Sinica》2021年第2期194-200,共7页力学学报（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China(Grants 12032010,11532009,11972185,11902155,and 12002156);the Natural Science Foundation of Jiangsu Province(Grant BK20190382);the Foundation of“Jiangsu Provincial Key Laboratory of Bionic Functional Materials”,China Postdoctoral Science Foundation(Grant 2020M671473);the Foundation for the Priority Academic Program Development of Jiangsu Higher Education Institutions.

摘　　要：Poroelasticity of cytoplasm is a rate-and size-dependent biphasic material behavior that reflects the normal activities and pathological states of cells,mainly caused by the migration of fluid molecules and the deformation of porous solid skeleton(protein scaffold).While micro/nano-indentation tests have been extensively used to characterize the poroelasticity of a cell,characterizing the in situ poroelasticity of cytoplasm remains elusive.In this study,based on the theory of the translation of a rigid spherical inclusion,we proposed a new method to characterize the in situ poroelasticity of cytoplasm.Based on data from optical/magnetic tweezers tests,we estimated three key poroelasticity parameters-shear modulus,Poisson ratio and diffusion coefficient-of cytoplasm for a variety of cells,including cardiomyocytes,endothelial cells of bovine capillary,and fibroblasts.The proposed method provides a powerful tool for in situ measurement of poroelastic properties of cytoplasm via optical/magnetic tweezers.

关 键 词：Biot theory Diffusion coefficient Optical/magnetic tweezers.Finite element 

分 类 号：O346.1[理学—固体力学]