基于UPLC-QTOF-MS技术分析多脂鳞伞特征性成分及体外抗肿瘤作用  

Chemical components of Pholiota adiposa analyzed by UPLC-QTOF-MS and their antitumor effects

作　　者：邢峻嘉 王晓岩 XING Jun-Jia;WANG Xiao-Yan(Medical Academy,Changchun Science-Technology University,Changchun,Jilin 130600,China;College of Traditional Chinese Medicine,Jilin Agricultural Science and Technology University,Changchun,Jilin 132109,China)

机构地区：[1]长春科技学院医药学院,吉林长春130600 [2]吉林农业科技学院中药学院,吉林长春132109

出　　处：《菌物学报》2021年第9期2423-2432,共10页Mycosystema

摘　　要：多脂鳞伞是一种较为珍贵的药食用真菌。对其化学成分及体外抗肿瘤作用进行了研究。通过UPLC-QTOF-MS法、电喷雾离子源(ESI)及负离子全扫描模式测定了人工栽培多脂鳞伞的化学成分组成;体外培养Hep G-2、A549、Hela及MCF-7,用不同质量浓度多脂鳞伞乙醇提物处理细胞,应用CCK-8法及流式细胞术进行细胞毒性测试,并应用流式细胞术选择Hep G-2细胞系以进一步评估其对Hep G-2细胞凋亡分析。结果表明:经UPLC-QTOF-MS分析,从多脂鳞伞子实体分析得到的化学成分与食药用菌成分数据库中的38种成分相吻合,其中棕榈酸与9E,12E-octadecadienoicacid含量最高,甾醇类化合物与多糖类化合物占比较高;多脂鳞伞乙醇提取物对Hep G-2细胞毒性最强,其浓度为50μg/m L时凋亡率能够达到(23.71±1.59)%。多脂鳞伞具有多种功能性化学成分,并具有潜在的抗肿瘤应用价值,初步断定其抗肿瘤活性是功能性成分通过诱导细胞凋亡实现的。The chemical constituents and anti-tumour activity in vitro of cultivated Pholiota adiposa were studied by using UPLC-QTOF-MS method.Hep G-2,A549,He La and MCF-7 cells were cultured in vitro and treated with different concentrations of ethanol extracts of Pholiota adiposa(EPA),and the cytotoxicity was assayed by CCK-8 method.Flow cytometry was used for Hep G-2 cells,apoptosis analysis via annexin VFITC/PI double staining.A total of 38 components is consistant with that recorded in the chemical composition database.The content of palmitic acid and 9 E,12 E-octadecadienoic acid was the highest,and sterol compounds and polysaccharide compounds accounted for a relatively high proportion.EPA showed the strongest cytotoxicity to Hep G-2 cells,and the apoptosis rate was up to(23.71±1.59)%under the concentration of 50μg/m L.Pholiota adiposa has a variety of functional chemical constituents and potential anti-tumor application value.It is preliminally concluded that the anti-tumor activity is realized by the functional constituents through inducing apoptosis.

关 键 词：多脂鳞伞 UPLC-QTOF-MS CCK-8法 细胞凋亡 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]