藜麦CAT基因家族的鉴定  

Identification of CAT Gene Family in Chenopodium quinoa

作　　者：孙敏 韩建玮 王美玲 张立培 张侠[1] 郭善利[1] 尹海波[1] Sun Min;Han Jianwei;Wang Meiling;Zhang Lipei;Zhang Xia;Guo Shanli;Yin Haibo(College of Life Science,Yantai University,Yantai,264005;Yantai Jien Biological Science&Technology Ltd.,Yantai,264006)

机构地区：[1]烟台大学生命科学学院,烟台264005 [2]烟台吉恩生物科技有限公司,烟台264006

出　　处：《分子植物育种》2021年第18期5933-5942,共10页Molecular Plant Breeding

基　　金：山东省“渤海粮仓”科技示范工程升级版计划项目(2019BHLC001);国家自然科学基金青年基金(31000128);烟台大学博士启动基金项目(TM09B25)共同资助。

摘　　要：为探讨过氧化氢酶(Catalase,CAT)在藜麦基因组中的结构和表达特点,分析CAT在藜麦耐受逆境胁迫机制中的作用,本研究从藜麦基因组中鉴定出4个可分为2个亚组的CAT基因家族成员。系统进化树显示藜麦CqCAT基因家族成员的基本特征在单双子叶植物分离之前就已形成。该家族成员的二级结构主要特点为:由α-螺旋和无规则卷曲组成;含20种以上氨基酸,存在10种不同的保守基序。基因启动子区域共预测到响应脱落酸、茉莉酸、水杨酸、低温和干旱等11种相关的顺式作用元件。RNA-seq分析表明该家族成员具有组织特异性表达。RT-qPCR分析表明CqCAT基因受NaCl和干旱胁迫诱导表达,同时CAT酶活性也表现为上调。推测藜麦CqCAT基因可能参与多种胁迫的响应过程,其高表达可能提高藜麦的耐盐能力,研究结果可为进一步研究藜麦CqCAT基因家族的功能提供依据。In order to investigate the structure and expression characteristics of catalase(CAT)in Chenopodium quinoa genome,and analyze the role of CAT in stress tolerance mechanism of quinoa,four CAT family genes were identified from quinoa genome,which can be divided into two subgroups.Phylogenetic tree showed that the basic characteristics of CqCAT gene family were formed before monocotyledons were isolated.The main characteristics of the secondary structure of the family members were as follows:composed ofα-helix and random coil;more than 20 kinds of amino acids and 10 different conserved motifs.A total of 11 cis-acting elements in this family were predicted to respond to abscisic acid,jasmonic acid,salicylic acid,low temperature and drought.RNA-seq analysis showed that the family members had tissue-specific expression.RT-qPCR analysis showed that the expression of CqCAT genes were induced by Na Cl and drought stress,and CAT activity was also up-regulated.It is speculated that quinoa CqCAT genes may be involved in the response process of various stresses,and the high expression may improve the salt tolerance of quinoa.The results could provide a basis for further study on the function of CqCAT family.

关 键 词：藜麦 过氧化氢酶 CAT基因家族 基因表达 

分 类 号：S519[农业科学—作物学]