贵州省优质水稻‘大粒香’香味基因分子标记的开发  

Development of Molecular Marker for Fragrance Gene of High Quality Rice’Dalixiang’in Guizhou Province

作　　者：邓力喜[1] 刘丹 陈银华[1] 冯章丽 龙秋红 Deng Lixi;Liu Dan;Chen Yihua;Feng Zhangli;Long Qiuhong(College of Biology and Agriculture,Zunyi Nomal University,Zunyi,563006)

机构地区：[1]遵义师范学院生物与农业科技学院,遵义563006

出　　处：《分子植物育种》2021年第18期6059-6063,共5页Molecular Plant Breeding

基　　金：贵州省教育厅自然科学研究项目(黔教合KY字[2015]413);贵州省高层次创新型人才培养项目(遵市科合人才[2017]18号);贵州省科技合作计划项目(黔科合LH字[2016]7013号);遵义师范学院博士基金(遵师BS[2014]15号);国家重点研发计划(2018YFD0200303)共同资助。

摘　　要：‘大粒香’香味形成是由Badh2基因第7个外显子的8 bp缺失和3 bp突变引起。为了建立了‘大粒香’香味基因的快速准确的检测方法,本研究根据‘大粒香’和‘日本晴’Badh2基因序列的差异,设计了7条引物组合成11对功能标记检测引物,通过对引物组合扩增的产物进行电泳检测,筛选出最佳的引物组合。试验结果表明,开发设计的引物能够准确的检测出香味基因,PCR反应的最佳退火温度为60℃,其中BADH2-F1+BADH2-R+badh2-R2引物组合能准确区分非香基因纯合体、香味基因纯合体以及杂合体三种基因型。基于该功能引物引物组合BADH2-F1+BADH2-R+badh2-R2可以快速开展香味基因的MAS育种。本研究结果将有助于利用Badh2基因位点的分子标记培育香型优质水稻品种,为改良贵州省水稻品种的香味品质提供了技术支撑。The fragrance of’Dalixiang’is caused by 8 bp delete and 3 bp mutant in the 7 thexon of Badh2 gene.A rapid and accurate detection method of fragrance gene in’Dalixiang’was established,whichbased on the sequence difference between’Dalixiang’and’Nipponbare’in Badh2 gene,11 pairs of functional molecular markers were designed which composed by 7 markers.And the best primers were selected by electrophoresis detection after the PCR products amplified thought primer combination.The results showed that primers which designed could detect the fragrance gene accurately,the PCR optimal annealing temperature was 60,and the primer combination of BADH2-F1+BADH2-R+badh2-R2 could be able to accurately identify three genotypes such as homozygous with non-fragrance gene,homozygous with fragrance gene and heterozygous.BADH2-F1+BADH2-R+badh2-R2 marker combination can quickly and accurately identify the fragrance gene,which is the basis of MAS breeding for fragrance gene.This study will be helpful to further breeding of aromatic rice varieties using molecular markers of Badh2 gene and provide technical support for improving aroma quality of Guizhou rice varieties.

关 键 词：水稻 香味基因 Badh2 功能分子标记 

分 类 号：S511[农业科学—作物学]