川渝地区野生鹅观草遗传多样性的ISAP分析  被引量:1

ISAP Analysis of Genetic Diversity of Wild Roegneria kamoji in Sichuan and Chongqing Area

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作  者:王萌[1] 罗丹[1] 易莉美 廖宗超 苏晏宁 夏嘉奇 张新全[1] 聂刚 Wang Meng;Luo Dan;Yi Limei;Liao Zongchao;Su Yanning;Xia Jiaqi;Zhang Xinquan;Nie Gang(College of Grassland Science and Technology,Sichuan Agricultural University,Chengdu,611130)

机构地区:[1]四川农业大学草业科技学院,成都611130

出  处:《分子植物育种》2021年第18期6248-6257,共10页Molecular Plant Breeding

基  金:国家现代牧草产业技术体系(CARS-34);四川农业大学本科生科研兴趣培养计划项目(NO.2018049);四川农业大学创新训练计划项目(S201910626072)共同资助。

摘  要:中国的西南地区具有丰富的牧草资源,其中野生鹅观草分布较为广泛,特别是在川渝地区。为了解川渝地区野生鹅观草遗传多样性及群体结构,为鹅观草种质资源搜集、评价及育种利用提供理论依据。本试验采用新型ISAP分子标记技术对80份来自于川渝地区野生鹅观草进行遗传多样性分析,结果表明20对引物共扩增出102条清晰可识别条带,多态性条带占93.14%。每个ISAP位点的PIC均值为0.255。聚类分析发现在GS为0.815处可将80份野生鹅观草种质资源分为3大类,材料间的聚类与地理来源呈一定的相关性。本研究结果将为今后鹅观草及小麦族类作物的遗传育种工作提供材料及理论基础。There are abundant forage resources in southwest China,among which the wild Roegneria kamoji Ohwi are widely distributed,especially in Sichuan and Chongqing province.Aim to figure out the genetic structure of wild R.kamoji Ohwi in these places,providing the theoretical basis for germplasm resources collection,utilization and breeding,also exploring the genetic diversity of wild R.kamoji Ohwi.In this experiment,the genetic diversity of 80 wild R.kamoji Ohwi from Sichuan and Chongqing were analyzed by the new molecular maker ISAP.Results showed that a total of 102 distinct and recognizable bands were amplified from 20 pairs of primers,with 97 polymorphic bands accounting for 93.14%.The PIC average value of each ISAP site was 0.272.The results of cluster analysis showed that the germplasm resources of 80 wild R.kamoji Ohwi were divided into 3 groups when GS was 0.815,and the clustering among the materials showed a certain correlation with geographical sources.This study might contribute to provide a theoretical basis and materials for the genetic breeding of R.kamoji Ohwi and wheat family crops in the future.

关 键 词:鹅观草(Roegniera kamoji Ohwi) ISAP 分子标记 遗传多样性 

分 类 号:S543.2[农业科学—作物学]

 

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