抗弧菌光合细菌的分离鉴定及对氨氮和亚硝态氮的降解特性  

Isolation and identification of vibrio resistant photosynthetic bacteria and degradation of nitrite nitrogen and ammonia nitrogen

作　　者：王雨婷 周荣翔 李霁虹 张瑶 周婷婷 陈文才 彭云 汤曼利 马桂珍 许建和 WANG Yuting;ZHOU Rongxiang;LI Jihong;ZHANG Yao;ZHOU Tingting;CHEN Wencai;PENG Yun;TANG Manli;MA Guizhen;XU Jianhe(School of Environmental and Chemical Engineering,Jiangsu Ocean University,Lianyungang 252000,China;School of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222000,China;Yongqing County Agricultural and Rural Bureau of Plantation Technology Extension Station,Langfang 065000,China;School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222000,China)

机构地区：[1]江苏海洋大学环境与化学工程学院,江苏连云港222000 [2]江苏海洋大学食品科学与工程学院,江苏连云港222000 [3]永清县农业农村局种植业技术推广站,河北廊坊065000 [4]江苏海洋大学海洋科学与水产学院,江苏连云港222000

出　　处：《南方水产科学》2021年第5期26-33,共8页South China Fisheries Science

基　　金：江苏省农业科技自主创新资金项目[CX(19)3107];江苏省研究生科研创新计划项目(KYCX20-2959)。

摘　　要：该研究采用双层平板涂布法和划线法,从不同地区的海洋环境样品中分离纯化光合细菌,以副溶血弧菌(Vibrio parahemolyticus)、创伤弧菌(V.vulnificus)、鳗弧菌(V.anguillarum)为指示菌,采用牛津杯法测定海洋光合细菌菌株的抑菌作用,采用靛酚蓝分光光度法和盐酸萘乙二胺分光光度法测定不同菌株对氨氮(NH_(4)^(+)-N)和亚硝态氮(NO_(2)^(−)-N)的降解作用,筛选出具有抗弧菌并高效降解NH_(4)^(+)-N和NO_(2)^(−)-N复合功能的优良菌株。结果显示,从30个海水、海泥等样品中分离得到3株光合细菌,分离自连云港车牛山岛海水样品的菌株P-3,对3种弧菌均具有较强的抑制作用,其中对鳗弧菌的作用最强,抑菌圈直径为5.3 mm。3株光合细菌均具有一定的降解NH_(4)^(+)-N和NO_(2)^(−)-N作用,菌株P-3的降解作用最强,在含有50 mg·L^(−1)NH_(4)^(+)-N和NO_(2)^(−)-N的培养基中培养4 d,降解率分别为89.68%和94.98%。经形态学观察、生理生化试验和16S rDNA序列分析,确定P-3为沼泽红假单胞菌(Rhodopseudomonas palustris)。In this study,photosynthetic bacteria(PSB)were isolated and purified from marine environmental samples from different areas by double-layer plate coating method and scribing method.Vibrio parahaemolyticus,V.vulnificus and V.anguillarum were used as control.The inhibition of marine photosynthetic bacteria was determined by Oxford cup method,and the degradation of nitrite nitrogen(NO_(2)^(−)-N)and ammonia nitrogen(NH_(4)^(+)-N)by different strains was determined by naphthalene ethylenediamine hydrochloride spectrophotometry and indophenol blue spectrophotometry.The results show that three strains of photosynthetic bacteria were isolated from 30 sea water and mud samples,and the P-3 strain isolated from the seawater samples of Cheniushan Island in Lianyungang had strong inhibitory effect on three kinds of Vibrio,especially for V.anguillarum,with the inhibition zone diameter of 5.3 mm.The results show that all the three photosynthetic bacteria have certain ability to degrade NO_(2)^(−)-N and NH_(4)^(+)-N,and P-3 strain had the strongest ability.The degradation rates of P-3 strain were 89.68%and 94.98%respectively,when being cultured in the medium containing 50 mg·L^(−1)NH_(4)^(+)-N and NO_(2)^(−)-N for 4 d.P-3 strain was identified as Rhodopseudomonas palustris by morphological observation,physiological and biochemical tests and 16S rDNA sequence analysis.

关 键 词：光合细菌 沼泽红假单胞菌 分离鉴定 拮抗作用 亚硝态氮降解 氨氮降解 

分 类 号：Q93-33[生物学—微生物学]