双氢青蒿素影响人胶质瘤细胞生物学特性的研究  

Effects of Dihydroartemisinin on biological characteristics of human glioma cells

作　　者：刘秀中 董金垚[2] 张燕燕[3] 师如意[4] Liu Xiuzhong;Dong Jinyao;Zhang Yanyan;Shi Ruyi(Department of General Surgery,Wuxiang County People′s Hospital,Shanxi 046300,China)

机构地区：[1]山西省武乡县人民医院普通外科,046300 [2]山西医科大学附属山西省肿瘤医院外科特需病区 [3]山西医科大学第一医院普通外科 [4]山西医科大学基础医学院医学细胞生物学与遗传学教研室

出　　处：《中国药物与临床》2021年第20期3361-3364,I0002,共5页Chinese Remedies & Clinics

基　　金：山西省应用基础研究项目(201601D202107);山西医科大学科技创新基金(01201309)。

摘　　要：目的观察中药制剂双氢青蒿素(DHA)对胶质瘤SHG44细胞生物学特性的影响。方法利用细胞增殖实验(MTT)法、trans-well法、流式细胞术、聚合酶链反应(PCR)等方法分别检测DHA对SHG44细胞在增殖、迁移、细胞周期及Claudin-1基因表达等方面的影响。结果SHG44细胞的增殖、迁移能力可受到DHA显著抑制;DHA可以显著影响细胞之间的紧密连接蛋白Claudin-1的表达水平,使其上调表达。结论DHA可以通过影响SHG44细胞生物学特性起到抗癌作用,是潜在的治疗胶质瘤的新药。Objective To determine the effects of Dihydroartmisinin(DHA),a traditional Chinese medical preparation,on the biological characteristics of glioma cell line SHG44.Methods MTT method,trans-well method,flow cytometry,PCR were used to determine the effects of DHA on the proliferation,migration,cell cycle and Claudin-1 gene expression of SHG44 cells,respectively.Results The proliferation and migration of SHG44 cells may be significantly inhibited by DHA.DHA may significantly affect the expression level of the tight junction protein Claudin-1 between cellsto up-regulate the expression.Conclusion DHA plays an anti-cancer effect by affecting the biological characteristics of SHG44 cells,and is a potential new drug for the treatment of glioma.

关 键 词：双氢青蒿素 胶质瘤 细胞生物学 

分 类 号：R285.5[医药卫生—中药学]