基于超连续谱光源的相干反斯托克斯拉曼散射成像理论与实验可行性研究  

Theoretical and Experimental Studies on Coherent Anti-Stokes Raman Scattering Microscopy Based on Supercontinuum Fiber Laser

作　　者：王浩宇 张娇娇 王楠 周王婷 徐欣怡 曾琦 陈雪利 WANG Haoyu;ZHANG Jiaojiao;WANG Nan;ZHOU Wangting;XU Xinyi;ZENG Qi;CHEN Xueli(School of Life Science and Technology,Xidian University,Xi'an 710071,China)

机构地区：[1]西安电子科技大学生命科学技术学院,西安710071

出　　处：《光子学报》2021年第10期105-116,共12页Acta Photonica Sinica

基　　金：中央高校基本科研业务费(No.QTZX2105);陕西省重点研发计划项目(No.2021ZDLSF04-05);国家重点研发计划精准医学专项课题(No.2018YFC0910600);国家自然科学基金(No.81871397);国家“万人计划”青年拔尖人才支持计划;陕西省杰出青年科学基金(No.2020JC-27);陕西省“特支计划”青年拔尖人才。

摘　　要：将超连续谱光纤激光器引入到相干反斯托克斯拉曼散射成像系统,以解决当前相干反斯托克斯拉曼散射成像系统造价昂贵、体积庞大的问题。首先分析了基于超连续谱光源的相干反斯托克斯拉曼散射成像理论及其影响因素;接着搭建了一套基于超连续谱光源的相干反斯托克斯拉曼散射成像系统,并进行了成像性能测试。在物镜、功率、浓度等不同影响因素的情况下,系统测试的实验结果与数值仿真结果具有高度一致性,验证了基于超连续谱光源的相干反斯托克斯拉曼散射成像理论与实验的可行性。A coherent anti-Stokes Raman scattering microscope is built by using a supercontinuum fiber laser,towards a solution of the disadvantages in current imaging system,such as the cost and bulk-size.Firstly,the theory of supercontinuum fiber laser-based coherent anti-Stokes Raman scattering imaging is deduced and its influencing factors are analyzed. An experimental setup for the supercontinuum fiber laserbased coherent anti-Stokes Raman scattering microscope is then built up,while its experimental feasibility is demonstrated. Different influencing factors,including objective lens,laser power and concentration,are selected to demonstrate that the test results are highly consistent with theoretical ones. The results prove the theoretical and experimental feasibility of coherent anti-Stokes Raman scattering microscopy based on supercontinuum fiber laser.

关 键 词：非线性光学 无标记显微成像 拉曼散射成像 相干反斯托克斯拉曼散射 显微成像 超连续谱光纤激光器 

分 类 号：O657.319[理学—分析化学]