荧光寿命成像在癌症诊断研究中的应用(特邀)  

Application of Fluorescence Lifetime Imaging in Cancer Diagnosis(Invited)

作　　者：刘立新 齐美捷 郜鹏 薛平[3] 屈军乐[4] LIU Lixin;QI Meijie;GAO Peng;XUE Ping;QU Junle(School of Physics and Optoelectronic Engineering,Xidian University,Xi'an 710071,China;CAS Key Laboratory of Spectral Imaging Technology,Xi'an 710119,China;State Key Laboratory of Low-Dimensional Quantum Physics,Department of Physics,Tsinghua University,Beijing 100084,China;Key Laboratory of Optoelectronic Devices and Systems,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen,Guangdong 518060,China)

机构地区：[1]西安电子科技大学物理与光电工程学院,西安710071 [2]中国科学院光谱成像技术重点实验室,西安710119 [3]清华大学物理系低维量子物理国家重点实验室,北京100084 [4]深圳大学物理与光电工程学院光电子器件与系统实验室,广东深圳518060

出　　处：《光子学报》2021年第10期267-289,共23页Acta Photonica Sinica

基　　金：国家自然科学基金(No.62075177);陕西省自然科学基金(Nos.2020JM-193,2020JQ-324);中国科学院光谱成像重点实验室开放基金(No.LSIT202005W);高等学校学科创新引智计划(No.B17035)。

摘　　要：癌症是目前人类所面对的共同难题,降低癌症死亡率的关键是实现早期诊断。荧光寿命因对微环境的敏感性,不仅可以实现对早期癌症组织与正常组织的区分,还可以对药物治疗癌症进行监测,因此荧光寿命成像显微技术在癌症诊断方面具有巨大的应用潜力。本文介绍了荧光寿命成像显微技术的基本原理及检测方法,总结了内源性和外源性荧光团的特征及其与癌症诊断的关系,综述了近年来荧光寿命成像显微技术在神经系统、呼吸系统、消化系统、生殖系统、泌尿系统、内分泌系统以及皮肤系统等癌症诊断中的应用,讨论了荧光寿命成像显微技术在癌症诊断中的应用优势、局限性以及未来发展趋势。Cancer is currently a common problem facing human beings.Early diagnosis is a key factor in reducing cancer mortality.Due to its sensitivity to the microenvironment,fluorescence lifetime imaging microscopy(FLIM)can not only distinguish early cancer tissues from normal tissues,but also monitor the drug treatment of cancer.Therefore,FLIM has great application potential in cancer diagnosis.This article introduces the basic principles and detection methods of FLIM,summarizes the endogenous and exogenous fluorescence characteristics and their relationship with cancer.In addition,this article mainly reviews the recent applications of FLIM in the diagnosis of cancers in the nervous system,respiratory system,digestive system,reproductive system,urinary system,endocrine system and skin system,and discusses the application advantages,limitations and future development trends of FLIM in cancer diagnosis.

关 键 词：荧光寿命 荧光寿命成像 癌症诊断 内源性荧光团 外源性荧光团 

分 类 号：O433[机械工程—光学工程] R73[理学—光学]