外周血NLRP3炎症小体表达和Th细胞亚群变化与类风湿关节炎患者的生活质量和关节功能的关联  

The relationship between the expression of NLRP3 inflammasome and the changes of The cell subsets in peripheral blood and the quality of life and joint function of patients with rheumatoid arthritis

作　　者：崔轶霞[1] 赵枫[2] 张娜娜 邹琳 CUI Yi-xia;ZHAO Feng;ZHANG Na-na(Department of Rheumatology and Immunology,Yan'an 716000,Shaanxi,China;Department of Pediatrics,Affiliated Hospital of Yan'an University,Yan'an 716000,Shaanxi,China;School of Medicine,Yan'an University,Yan'an Shaanxi 716000,China)

机构地区：[1]延安大学附属医院风湿免疫科,陕西延安716000 [2]延安大学附属医院儿科,陕西延安716000 [3]延安大学医学院,陕西延安716000

出　　处：《临床和实验医学杂志》2021年第16期1716-1719,共4页Journal of Clinical and Experimental Medicine

基　　金：陕西省教育厅科研计划项目(编号:09JK825);延安大学附属医院科学技术研究发展计划项目(编号:2018PT-08)。

摘　　要：目的探讨外周血亮氨酸富集的核苷酸结合寡聚结构域(NLRP)-3炎症小体表达和Th细胞亚群变化与类风湿关节炎患者的生活质量和关节功能的关联性。方法回顾性选择2018年7月到2021年2月延安大学附属医院风湿免疫科收治的类风湿关节炎患者134例作为病例组,同期选择健康体检人134例作为对照组。检测2组血清NLRP3、白细胞介素(IL)-2、IL-17、1,25-二羟维生素D3[25-(OH) D3]含量与Th细胞亚群比例,调查2组的生活质量、关节功能并进行相关性分析。结果病例组的血清NLRP3、IL-2、IL-17水平为(98.24±3.18)、(31.94±2.49)、(30.44±3.98) pg/mL,显著高于对照组[(67.10±9.14)、(9.22±1.84)、(15.53±2.98) pg/mL],血清25-(OH) D3水平(15.29±3.18) ng/mL,显著低于对照组[(45.22±2.18) ng/mL],差异均有统计学意义(P<0.05)。病例组的Th2、Th17细胞比例分别为(23.38±2.04)%、(29.93±5.64)%,高于对照组[(17.36±3.50)%、(21.02±3.11)%],Th1细胞比例为(20.34±3.15)%,低于对照组[(25.62±4.11)%],差异均有统计学意义(P<0.05)。病例组的生活质量评分(78.76±3.15)分,低于对照组[(91.92±2.77)分],DAS28评分(6.55±1.30)分,高于对照组[(2.59±0.22)分],差异均有统计学意义(P<0.05)。病例组Pearson相关性显示NLRP3、Th1细胞、Th2细胞、Th17细胞比例与生活质量评分、DAS28评分均存在相关性(P<0.05)。结论类风湿关节炎患者多伴随有外周血NLRP3炎症小体高表达和Th细胞亚群紊乱,与患者的生活质量和关节功能均存在相关性。Objective To investigate the relationship between the expression of nucleotide-binding oligomerization domain-like receptor protei(NLRP)-3 inflammasomes and the changes of Th cell subsets in peripheral blood and the quality of life and joint function of patients with rheumatoid arthritis. Methods From July 2018 to February 2021,134 patients with rheumatoid arthritis admitted to the Department of Rheumatology and Immunology in Affiliated Hospital of Yan’an University were selected as the case group,and 134 cases of healthy persons were selected as the control group during the same period. The serum NLRP3,interleukin(IL)-2,IL-17,1,25-dihydroxyvitamin D3[25-(OH) D3]content and Th cell subpopulation ratio of the two groups were tested,and were to investigate the quality of life and joint function of the two groups and given correlation analysis. Results The levels of serum NLRP3,IL-2 and IL-17 in the case group were(98. 24 ± 3. 18),(31. 94 ± 2. 49),(30. 44 ±3. 98) pg/mL,respectively,which were significantly higher than those in the control group [(67. 10 ±9. 14),(9. 22 ±1. 84),(15. 53 ±2. 98) pg/mL],the level of serum 25-(OH) D3 was(15. 29 ± 3. 18) ng/mL,which was significantly lower than that in the control group[(45. 22 ± 2. 18) ng/mL],the differences were statistically significant(P<0.05). The proportions of Th2 and Th17 cells in the case group were(23. 38 ± 2. 04) % and(29. 93 ± 5. 64) %,respectively,which were higher than those in the control group [(17. 36 ± 3. 50) %,(21. 02± 3. 11) % ],and the proportion of Th1 cells was(20. 34 ± 3. 15) %,which was lower than that in the control group [(25. 62 ± 4. 11) % ],the differences were statistically significant(P<0.05). The quality of life score in the case group were(78. 76 ± 3. 15) points,which were lower than those in the control group [(91. 92 ± 2. 77) points],and the DAS28 score was(6. 55 ± 1. 30) points,which was higher than that in the control group [(2. 59 ± 0. 22) points],the differences were statistical significance(P<0.05). In the case gr

关 键 词：类风湿关节炎 NLRP3炎症小体 TH细胞亚群 生活质量 关节功能 

分 类 号：R593.22[医药卫生—内科学]