砂仁复方对5-FU所致大鼠肠道菌群调节作用研究  

The Effect of Amomum Villosum Compound on the Changes of Intestinal Function in Rats Induced by 5-FU

作　　者：李菊 赵荣华 陈鑫 何玉潇 牛璐璐 孙瑞芬 LI Ju;ZHAO Ronghua;CHEN Xin;HE Yuxiao;NIU Lulu;SUN Ruifen(Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学,云南昆明650500

出　　处：《云南中医学院学报》2021年第2期14-20,共7页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81560429);南药研究协同创新中心专项基金研究项目(2011YXZD07)。

摘　　要：目的探讨砂仁复方对5-FU所致大鼠肠道菌群失衡的影响。方法将SD大鼠随机分为正常组、阳性组、模型组、砂仁复方高、中、低剂量组;通过对大鼠粪便细菌16S rRNA测序,i-Singer云平台数据分析,对肠道菌群物种多样性、不同分类学水平菌群丰度进行分析。结果砂仁复方可以增加模型组肠道菌群多样性;增加乳杆菌科等的丰度;降低普雷沃氏菌科等的丰度,并以低剂量效果最佳。结论砂仁复方可以通过平衡肠道菌群,增加菌群多样性,并在不同分类水平下调节肠道菌群稳态来发挥肠道保护作用。Objective To investigate the effect of Amomum villosum compound on intestinal flora imbalance induced by chemotherapeutic drug 5-FU in rats.Methods The Spraque-Dawley(SD)rats were randomly divided into 6 groups,namely normal group,model group,positive drug group,and Amomum villosum low,medium and high dose groups.Based on the 16S rRNA sequencing of rat fecal bacteria and the analysis of i-Singer cloud platform data,the species diversity of intestinal flora and the abundance of flora at different taxonomical levels were analyzed.Results The results show that Amomum villosum compound can increase the diversity of intestinal flora in model group rats,and increase the abundance of Lactobacillaceae,et al;and decreased the abundance of Prevotellaceae,et al,and the best effect was obtained with the low dose of Amomum villosum compound.Conclusion Amomum villosum compound can increase the diversity of intestinal flora and regulate the homeostasis of intestinal flora at different classification levels to play an intestinal protective role.

关 键 词：5-FU 砂仁复方 肠道粘膜屏障 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]