昆明地区脂肪肝痰瘀证候ZJU指数及基因多态性研究  

Research of ZJU-Related Factors and Genetic Polymorphism of Phlegm-Stasis Syndrome Fatty Liver in Kunming

作　　者：杨艳 洪力 韦姗姗 姚政 季能博 徐寻 吕一航 王维 YANG Yan;HONG Li;WEI Shanshan;YAO Zheng;JI Nengbo;XU Xun;LV Yihang;WANG Wei(Yunnan University of Chinese Medicine,Kunming 650050,China;Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine,Kunming 650050,China;Key Laboratory of Microcosmic Syndrome Differentiation,Kunming 650050,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南省中医药学分子生物学重点实验室,云南昆明650500 [3]云南省高校中医证候微观辨证重点实验室,云南昆明650500

出　　处：《云南中医学院学报》2021年第2期30-35,共6页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金地区项目(81960816);云南省科技计划项目青年项目(2017FD112);云南省科技厅科技计划项目中医联合面上项目[2018FF001(-014)];云南省科技厅科技计划项目中医联合青年项目[2018FF001(-083)]。

摘　　要：目的分析昆明地区脂肪肝(FLD)患者糖脂代谢相关基因多态性,探究该地区脂肪肝痰瘀证候与基因多态性之间的关系,进而探讨从基因层面优化ZJU指数,并将其应用于FLD临床辨证分型的可能性。方法以40例FLD痰瘀证型患者为病例组,无脂肪肝者40例为对照组,对总共80例样本测量身高、体质量,检测肝功能、血糖、血脂等血生化指标;同时进行肝脏B超检查,后期检测APN rs2241766、APN rs1501299、LEP rs7799039、LEPR rs1137100、PNPLA3 rs7384095个基因多态性位点。结果本次调查的ZJU指数显示对照组(33.26±2.83)与病例组(37.82±4.97)差异具有统计学意义(P<0.05)。对相关基因SNP位点进行检测后发现,LEPR rs1137100(K109R)和PNPLA3 rs738409(I148M)基因多态性位点在两组间存在差异(P<0.05),其余基因多态性位点分布在两组间无显著差异。结论LEPR rs1137100(K109R)和PNPLA3 rs738409(I148M)两个基因多态性位点的差异可能在FLD痰瘀证型形成过程中发挥重要作用,这两个基因可能是中医痰瘀证候的生物学基础之一。Objective The biological basis of phlegm and blood stasis related syndromes was explained by exploring the SNPs of glucose and lipid metabolism related genes in patients with fatty liver in Kunming,and the correlation between ZJU related indexes and the syndromes of phlegm and blood stasis.According to this research,it provides the basis for the objectification of syndrome differentiation.Methods Forty cases of FLD were divided into phlegm and blood stasis syndrome as experimental groups,forty cases healthy people as the control group.Height,weight,liver function,FPG,TG,liver B-mode ultrasonography,SNPs of sugar and lipid metabolism related genes were tested.Results The ZJU index in this research showed that there was a significant difference between FLD group(37.82±4.97)and contrast(33.26±2.83)(P<0.01).As the SNPs of sugar and lipid metabolism related genes were detected,the difference between LEPR rs1137100(K109R)and PNPLA3 rs738409(I148M)was found.But there was not directly correlation between genotypes and occurrence of FLD.Conclusion Difference in SNPs of LEPR rs1137100(K109R)and PNPLA3 rs738409(I148M)may play an important role in the aggravation of phlegm-stasis related syndromes.The two genes may be one of the biological bases of phlegm-stasis related syndromes .

关 键 词：脂肪肝 痰瘀证型 ZJU指数 基因多态性 

分 类 号：R259[医药卫生—中西医结合] R575.5[医药卫生—中医内科学]