NEFA对奶牛中性粒细胞跨乳腺上皮迁移功能的影响  

Effects of NEFA on neutrophils trans-mammary epithelial migration in dairy cows

作　　者：吕观新 王海 武瑞[1] LYU Guanxin;WANG Hai;WU Rui(Laboratory of Veterinary Clinical Diagnosis and New Veterinary Drug Research,College of Animal Science and Technology,Heilongjiang Bayi Agriculture University,Daqing,Heilongjiang 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院兽医临床诊断与新兽药研发实验室,黑龙江大庆163319

出　　处：《中国兽医学报》2021年第9期1780-1784,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31272624)。

摘　　要：为了体外研究游离脂肪酸(NEFA)对奶牛多形核中性粒细胞(PMN)跨奶牛乳腺上皮迁移功能的影响,用Transwell嵌套构建了跨乳腺上皮迁移模型,并以此模型研究了NEFA对PMN跨乳腺上皮迁移功能的影响。结果显示,不同浓度的油酸(OA)可显著促进PMN跨乳腺上皮迁移数量(P<0.01),其作用特点呈剂量依赖效应;棕榈酸(PA)及硬脂酸(SA)对PMN跨乳腺上皮迁移数量并无显著影响(P>0.05)。结果表明,NEFA不同组分对奶牛PMN迁移功能具有不同的调控作用,通过改善饲料成份和配比提高OA在奶牛体内NEFA中的占比,可能有助于防治奶牛乳腺炎。In order to investigate the effects of NEFA on the migration of neutrophils across the mammary in vitro,MAC-T were cultured in transwell basement membrane which was used as a trans-epithelial migration model and the effect of NEFA on the transepithelial migration of PMN was studied.The results showed that different concentrations of oleic acid(OA)could significantly promoted the PMN trans-epithelial migration in a dose-dependent manner(P<0.01),palmitic acid(PA)and stearic acid(SA)had no effect on the PMN trans-epithelial migration(P>0.05),suggesting that different components of NEFA have different regulatory effects on PMN migration function in dairy cows.It may be helpful to prevent and control mastitis by improving feed composition and increasing the proportion of OA in NEFA.

关 键 词：中性粒细胞 TRANSWELL 游离脂肪酸 细胞迁移 

分 类 号：S852.2[农业科学—基础兽医学]