不同氮源对淡水多甲藻生长和叶绿素荧光参数的影响  

Effects of Different Nitrogen Sources on Growth and Chlorophyll Fluorescence Parameters of Freshwater Dinoflagellate,Peridinium umbonatum

作　　者：杜雨欣 代潇潇 杨燕君 施军琼 DU Yuxin;DAI Xiaoxiao;YANG Yanjun;SHI Junqiong(School of Life Sciences, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China)

机构地区：[1]西南大学生命科学学院/三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2021年第10期38-44,共7页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(41877410);重庆市研究生科研创新项目(CYS21106,CYS20105);西南大学大学生创新创业训练项目(X201910635050).

摘　　要：以淡水多甲藻(Peridinium umbonatum)为材料,以氯化铵、硝酸钠和尿素为氮源,在119培养基中培养,并测定有关生长和生理指标,研究不同氮源对淡水多甲藻生长和叶绿素荧光参数的影响.结果表明:①以氯化铵、硝酸钠和尿素为单一氮源的多甲藻,细胞密度显著高于无氮处理组(p<0.05),并且在尿素环境中具有更高的生物量和比生长率.②无氮处理使得多甲藻相对可变荧光值(Vj)显著升高(p<0.05).③在延滞期时,以硝酸钠、尿素为氮源的多甲藻捕获的激子将电子传递到电子传递链中超过QA的其他电子受体的概率(Ψ0)和性能指数(PIABS)显著高于无氮和氯化铵处理组;对数期时,有氮处理组Ψ0显著高于无氮处理组(p<0.05),且硝酸钠和氯化铵处理组显著高于尿素处理组;在平台期时,多甲藻单位面积吸收的光能(ABS/CS0)、单位面积捕获的光能(TR0/CS0)、单位面积电子传递的量子产额(ET0/CS0)、单位面积传递到电子传递链末端的能量(RE0/CS0)、单位面积内反应中心的数量(RC/CS0)以及Ψ0表现出相同的趋势,其值由大到小均为硝酸钠处理组、氯化铵处理组、尿素处理组、无氮处理组,且各处理组间差异有统计学意义(p<0.05).相比较而言,多甲藻在以氯化铵、硝酸钠和尿素为单一氮源时均可以生长良好,其中尿素最适合多甲藻生长,硝酸钠最利于多甲藻进行光合作用,而无氮处理(氮胁迫)时,多甲藻生长受到显著抑制,光合系统Ⅱ(PSⅡ)反应活性中心的数量降低,电子传递受阻.In this study,the growth and the chlorophyll fluorescence parameters of freshwater dinoflagellate,Peridinium umbonatum,were investigated when it was cultured in 119 medium with NH4Cl,NaNO3 and CO(NH2)2 as a single nitrogen source,respectively.The results show that①The cell density of P.umbonatum was significantly higher in the treatment of NH4Cl,NaNO3 and CO(NH2)2 as a single nitrogen source,respectively,than that of nitrogen-free treatment(p<0.05).Among the three treatments of nitrogen,a highest biomass and highest growth rate were determined at the treatment of CO(NH2)2;②The relative variable fluorescence(Vj)of P.umbonatum increased significantly under the nitrogen-free condition(p<0.05);③In the lag growth phase,the probability that a trapped exit on moves an electron into the electron transport chain beyond QA(Ψ0)and performance index(PIABS)increased significantly at the groups of NaNO3 and CO(NH2)2 in contrast with the groups of NH4Cl and nitrogen-free.In the logarithmic growth phase,however,theΨ0 of the groups of NH4Cl,NaNO3 and CO(NH2)2 increased significantly than that at the nitrogen-free treatment,andΨ0 in the groups of NaNO3 and NH4Cl was significantly higher than that at the group of CO(NH2)2.In the stable growth phase,absorption flux per CS(ABS/CS0),trapped energy flux per CS(TR0/CS0),electron transport flux per CS(ET0/CS0),energy transferred to the end of the electron transport chain per CS(RE0/CS0),the number of reactive centers(RC/CS0)andΨ0 showed a similar trend that a significant difference among the four groups(p<0.05),and highest values were found at the treatment of NaNO3,following as NH4Cl,CO(NH2)2 and nitrogen-free.As a whole,it is suggested that P.umbonatum could grow better at CO(NH2)2 than that at NH4Cl and NaNO3,while NaNO3 was the most beneficial nitrogen source for the photosynthesis of P.umbonatum compared with other nitrogen sources.Additionally,nitrogen deficiency significantly inhibited the growth of P.umbonatum because the number of reactive centers in PSⅡand the elect

关 键 词：氮源 多甲藻 光合系统Ⅱ 叶绿素荧光参数 

分 类 号：Q948[生物学—植物学]