滚环扩增技术在病原微生物检测中的应用  

Application of Rolling Ring Amplification in the Detection of Pathogenic Microorganism

作　　者：张世佳 冯建军 郭松林 王艺磊 林鹏 ZHANG Shi-jia;FENG Jian-jun;GUO Song-lin;WANG Yi-lei;LIN Peng(Engineering Research Centre of Eel Modern Technical Industry,Ministry of Education,Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture,Fisheries College,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,鳗鲡现代产业技术教育部工程研究中心,农业部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《分析测试学报》2021年第10期1538-1544,共7页Journal of Instrumental Analysis

基　　金：福建省自然科学基金(2017J01637);国家自然科学基金(31272685)。

摘　　要：滚环扩增(RCA)技术是一种简单的恒温DNA扩增技术,在DNA聚合酶的催化下通过扩增闭合环状模板产生成千上万的重复序列。相较于变温核酸扩增技术如聚合酶链式反应(PCR),RCA无需昂贵的变温仪器,更适合现场检测。该文介绍了RCA技术的原理和分类,综述了其在细菌、病毒以及其它病原微生物检测方面的应用现状,并展望了RCA检测病原微生物的应用前景。RCA在检测病原微生物领域有着巨大潜力,同时可为新型冠状病毒(SARS-CoV-2)的快速检测提供思路和补充。Rolling circle amplification(RCA)is a simple and isothermal DNA amplification tech⁃nique that is used to generate thousands of repeating DNA sequences using circular templates under the catalysis of DNA polymerase.Compared to alternating temperature nucleic acid amplification such as polymerase chain reaction(PCR)amplification,RCA is more suitable for on-spot detection with no need of the expensive thermal circulation meter.In this paper,the principle and classifica⁃tion of RCA are introduced,and the applications of RCA in the detections of bacteria,viruses and other pathogenic microorganisms are reviewed.Finally,the perspectives of RCA in the detection of pathogenic microorganisms are anticipated.The RCA method has great potentials in the detection of pathogenic microorganisms.Furthermore,it also could be used in the detection of severe acute re⁃spiratory syndrome coronavirus 2.

关 键 词：滾环扩增 锁式探针 病原微生物 检测 

分 类 号：R37[医药卫生—病原生物学]