体外全胚胎培养技术及其在药物生殖发育毒性评价中的应用  

Whole Embryo Culture Technique in Vitro and Its Applications in the Evaluation of Drug Reproductive and Developmental Toxicity

作　　者：朱晨霞 宋翼升 张立将 ZHU Chenxia;SONG Yisheng;ZHANG Lijiang(Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou 310014,China;Center of Safety Evaluation,Hangzhou Medical College(Zhejiang Academy of Medical Sciences),Hangzhou 310053,China)

机构地区：[1]浙江工业大学长三角绿色制药协同创新中心,杭州310014 [2]杭州医学院(浙江省医学科学院)安全性评价研究中心,杭州310053

出　　处：《中国现代应用药学》2021年第17期2162-2169,共8页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省重点研发计划项目(2020C03053,2021C03077);浙江省实验动物科技计划项目(2018C37127);浙江省医学科学院青年基金。

摘　　要：体外全胚胎培养(whole embryo culture,WEC)技术是一项在受控的体外环境中培养动物胚胎的技术,是研究药物及化学物质在胚胎发育过程中的药理毒理及作用机制的重要方法之一。本文从大鼠和兔的WEC技术发展概况、培养条件、胚胎发育终点的评分系统和拓展的胚胎发育评价终点、药物生殖发育毒理学应用等方面,对WEC技术及近年来研究进展进行介绍。WEC技术是药物生殖发育毒性研究中重要的替代学研究方法之一。其中,对大鼠与兔组合的WEC研究更具有重要价值,可为胚胎发育毒性研究提供更全面的信息。Whole embryo culture(WEC) technique in vitro is one of the most important methods to study the pharmacological toxicology and mechanism of drugs and chemicals during embryonic development, which is used to cultivate animal embryos in a controlled in vitro environment. In this paper, WEC technique and its recent research progress were reviewed from the perspectives of the development of rats and rabbits WEC, the culture conditions, the scoring system for embryonic development endpoints and the expanded embryonic development evaluation endpoints, and the applications of drug reproduction and developmental toxicology. WEC technique is one of the important alternative research methods in the research of drug reproduction and developmental toxicity, and the WEC study of the combination of rats and rabbits is more important and provides more comprehensive information for the study of embryonic developmental toxicity.

关 键 词：体外 全胚胎培养 胚胎发育 生殖毒性 安全性评价 

分 类 号：R99[医药卫生—毒理学]