Flexible,video-rate,and aberration-compensated axial dual-line scanning imaging with field-of-view jointing and stepped remote focusing  

作　　者：RUI JIN YALAN YU DAN SHEN QINGMING LUO HUI GONG JING YUAN 

机构地区：[1]Britton Chance Center for Biomedical Photonics and MoE Key Laboratory for Biomedical Photonics,Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan 430074,China [2]Current address:Department of Mechanical and Automation Engineering,Chinese University of Hong Kong,Shatin,Hong Kong [3]HUST-Suzhou Institute for Brainsmatics,JITRI Institute for Brainsmatics,Suzhou 215123,China [4]School of Biomedical Engineering,Hainan University,Haikou 570228,China

出　　处：《Photonics Research》2021年第8期1477-1485,共9页光子学研究（英文版）

基　　金：National Natural Science Foundation of China(61890950,61890953,91749209);Fundamental Research Funds for the Central Universities(2019kfy XMBZ039)。

摘　　要：Parallel dual-plane imaging with a large axial interval enables the simultaneous observation of biological structures and activities in different views of interest.However,the inflexibility in adjusting the field-of-view(FOV)positions in three dimensions and optical sectioning effects,as well as the relatively small effective axial range limited by spherical aberration,have hindered the application of parallel dual-plane imaging.Herein,we propose a flexible,video-rate,and defocus-aberration-compensated axial dual-line scanning imaging method.We used a stepped mirror to remotely generate and detect dual axial lines with compensation for spherical aberration and FOV-jointing to rearrange into a head-to-head line for high-speed optical sectioning acquisition.The lateral and axial positions of the two FOVs could be flexibly adjusted before and during imaging,respectively.The method also allows the adjustment of optical sectioning effects according to specific experimental requirements.We experimentally verified the consistent imaging performance over an axial range of 300μm.We demonstrated high throughput by simultaneously imaging Brownian motions in two 250μm×250μm FOVs with axial and lateral intervals of 150μm and 240μm,respectively,at 24.5 Hz.We also showed potential application in functional imaging by simultaneously acquiring neural activities in the optic tectum and hindbrain of a zebrafish brain.The proposed method is,thus,advantageous compared to existing parallel dual-plane imaging and potentially facilitates intravital biological study in large axial range.

关 键 词：AXIAL field ABERRATION 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] O439[自动化与计算机技术—计算机科学与技术]