慢性乙型肝炎患者血清IL-7与细胞免疫相关性研究  

Correlation analysis of serum IL-7 with evaluation of cellular immunity in chronic hepatitis B

作　　者：王珍子 王铁山[1] Wang Zhenzi;Wang Tieshan(Clinical Laboratory Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院临床检验中心,100050

出　　处：《中华微生物学和免疫学杂志》2021年第9期661-666,共6页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究慢性乙型肝炎(乙肝)患者血清IL-7与IL-21、乙型肝炎病毒(HBV)特异性细胞毒性T淋巴细胞(CTLs)、HBV DNA载量、T淋巴细胞表面CD127表达的关系,探讨IL-7对细胞免疫的影响。方法收集2017年9月至2020年3月至北京友谊医院就诊的560例慢性乙肝患者血清标本,采用ELISA法检测IL-7和IL-21;流式细胞仪检测HBV特异性CTL和T淋巴细胞表面CD127表达;实时荧光定量PCR(qRT-PCR)法检测HBV DNA。根据IL-7检测结果(低、中、高),将研究对象分为A、B、C 3组(A组:IL-7<20 pg/ml;B组:20 pg/ml≤IL-7<30 pg/ml;C组:IL-7≥30 pg/ml)。结果慢性乙肝患者血清IL-7平均浓度显著低于健康对照组(P<0.01),3组检测值差异有统计学意义(P<0.01),同时3组患者IL-21、HBV特异性CTL百分比、CD8+T淋巴细胞表面CD127的表达率也依次升高,3组检测值差异均有统计学意义(P<0.01),两两变量之间均呈正相关(P<0.01)。只有HBV DNA在A、B、C 3组呈下降趋势,3组检测值差异有统计学意义(P<0.01),与其他变量均呈负相关(P<0.01)。多重线性回归分析显示HBV特异性CTL是对HBV DNA回归分析的独立影响因素(P<0.01);IL-7、CD8+T淋巴细胞CD127表达、IL-21均是对HBV特异性CTL回归分析的独立影响因素(P<0.05)。结论慢性乙肝患者IL-7调节HBV特异性免疫应答,或可成为评价患者细胞免疫状态的有效细胞免疫指标。Objective To explore the possible correlation between serum detection of IL-7,IL-21,HBV-specific cytotoxic T lymphocytes(CTLs),HBV DNA,and the expression of CD127 on the T lymphocytes,and discuss the effect of IL-7 to cellular immune response in patients with chronic hepatitis B(CHB).Methods Five hundred and sixty serum samples were collected from patients with CHB in Beijing Friendship Hospital from September 2017 to March 2020.The serum IL-7 and IL-21 were detected by enzyme-linked immunosorbent assay(ELISA),and HBV-specific CTLs and the expression of CD127 on the T lymphocytes were determined by flow cytometry.While HBV DNA were tested using quantitative real-time PCR(qRT-PCR).Subjects were divided into groups A,B,and C,according to the IL-7 levels(low:IL-7<20 pg/ml,medium:20 pg/ml≤IL-7<30 pg/ml,and high:IL-7≥30 pg/ml).Results The average concentration of serum IL-7 in patients with CHB was significantly lower than that of healthy controls(P<0.01),and the difference among three groups was statistically significant(P<0.01).Meanwhile,levels of IL-21,percentages of HBV-specific CTL,and the expression of CD127 on the CD8+T lymphocytes showed an upward trend among groups,and there were significant differences among three groups(P<0.01)with a positive correlation between each two variables(P<0.01).However,HBV DNA showed a downward trend in group A,B and C,and the difference of the three groups were statistically significant(P<0.01),which were negatively correlated with other variables(P<0.01).Multiple linear regression analysis showed that HBV-specific CTL was an independent influencing factor for HBV DNA(P<0.01),and IL-7,the expression of CD127 on the CD8+T lymphocytes and IL-21 had an independent effect on HBV-specific CTL(P<0.05).Conclusions IL-7 could regulate HBV-specific immune response,and might be used as an effective cellular immune indicator to evaluate the cellular immune status of patients with chronic hepatitis B.

关 键 词：肝炎病毒 乙型 IL-7 IL-21 HBV特异性CTL DNA CD127 

分 类 号：R512.62[医药卫生—内科学]