百日咳鲍特菌的基因多样性及分子流行病学分析  

Genetic diversity and molecular epidemiology of Bordetella pertussis isolates

作　　者：张娟胜 王小强[1] 张弟强[2] 李浩[1] 魏晓光[1] 王增国[1] 刘莹[1] 赵悦宛 Zhang Juansheng;Wang Xiaoqiang;Zhang Diqiang;Li Hao;Wei Xiaoguang;Wang Zengguo;Liu Ying;Zhao Yuewan(Microbiological Laboratory,Xi′an Center for Disease Control and Prevention,Xi′an 710054,China;Department of Obstetrics and Gynecology,Pingchuan District People′s Hospital,Baiyin 730900,China)

机构地区：[1]西安市疾病预防控制中心微生物检验科,710054 [2]白银市平川区人民医院妇产科,730900

出　　处：《中华微生物学和免疫学杂志》2021年第9期687-691,共5页Chinese Journal of Microbiology and Immunology

基　　金：西安市科技计划项目(201805101YX9SF35(2));西安卫健委卫生科研人才培训项目(J201801016)。

摘　　要：目的探讨百日咳鲍特菌临床流行株的基因多样性分子流行病学特征,并分析该地区百日咳复现的可能原因。方法采用多位点抗原序列分型(MAST)和多位点可变数目串联重复序列分析(MLVA)对2012—2017年的临床分离株进行基因多样性和分子流行病学特征分析,并与疫苗株进行比较。结果流行株与疫苗株的分子型别特征不同。95%的流行株抗原基因型为prn1/ptxP1/ptxA1/fim3-1/fim2-1;8种MLVA型别中MT195(34%)、MT55(30%)和MT104(26%)占主导地位,其中MT195分离率连年增长。结论陕西省当前流行百日咳菌株基因特征与疫苗株不同,流行株通过基因变异发生进化可能是近年来该地区百日咳复现的原因之一。Objective To investigate the genetic diversity and molecular epidemiology of Bordetella pertussis in Shaanxi province,and analyze the possible reasons of resurgence in this region.Methods We characterized clinical isolates collected during 2012-2017 using multilocus antigen sequence typing(MAST)and multilocus variable-number tandem repeat analysis(MLVA).Results The circulating strains and vaccine strains were different in molecular characteristics.The majority(95%)of the isolates were typed as prn1/ptxP1/ptxA1/fim3-1/fim2-1.In addition,eight MLVA types(MTs)and eight PFGE profiles were identified,respectively.MT195,MT55 and MT104 were dominant and MT195 continually increased annually.Conclusions The genetic characteristics of the current strains in Shaanxi province were different from those of the vaccine strain.The evolution through genetic variation might be one of the reasons for the recurrence of pertussis in this region.

关 键 词：百日咳鲍特菌 基因多样性 分子流行病学 多位点抗原序列分型(MAST) 多位点可变数目串联重复序列分析(MLVA) 

分 类 号：R725.1[医药卫生—儿科] R181.3[医药卫生—临床医学]