2019年广州本地登革热病例血清型分布及病毒全基因组进化分析  

Serotype distribution and phylogenetic analysis of virus complete genome from indigenous dengue patients in Guangzhou in 2019

作　　者：赵令斋[1] 张璐[1] 洪文昕[2] 邱爽[2] 冯嘉敏 谭行华[2] 张复春[2] Zhao Lingzhai;Zhang Lu;Hong Wenxin;Qiu Shuang;Feng Jiamin;Tan Xinghua;Zhang Fuchun(Institute of Infectious Diseases,Guangzhou Eighth People′s Hospital,Guangzhou Medical University,Guangzhou 510060,China;Department of Infectious Diseases,Guangzhou Eighth People′s Hospital,Guangzhou Medical University,Guangzhou 510060,China)

机构地区：[1]广州医科大学附属市八医院传染病研究所,510060 [2]广州医科大学附属市八医院感染科,510060

出　　处：《中华微生物学和免疫学杂志》2021年第9期698-703,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家科技重大专项(2018ZX10102-001-005-002,2018ZX10101-001-005-002);广州市健康医疗协同创新重大计划项目(201803040006)。

摘　　要：目的对2019年广州本地登革热病例血清型分布和病毒全基因组进化特征进行分析,为登革热防治提供科学依据。方法应用登革病毒血清型特异性荧光PCR试剂盒进行分型,操作步骤按照试剂盒说明书严格进行;分离培养的病毒采用Illumina平台进行全基因组序列测定;从ViPR网站下载部分代表性序列,用MEGA7.0软件进行病毒系统发育分析。结果2019年广州本地登革热为3个血清型共流行,其中登革1型占比80.35%,2型占比12.97%,3型占比6.68%;3个血清型之间患者的性别、年龄分布和重症发生率差异均无统计学意义。病毒全基因组进化分析显示,登革1型分离株属于基因Ⅰ型,来源上有两个分支,与柬埔寨来源毒株亲缘关系近;登革2型分离株为Cosmopolitan型,与东南亚流行株亲缘关系近;登革3型分离株属于基因Ⅲ型,与印度病毒株在同一分支。结论2019年广州登革病毒为1、2和3三个血清型共流行,每一个血清型病毒分属同一种基因型。Objective To investigate the serotype distribution and phylogenetic analysis of virus complete genome from indigenous dengue patients in Guangzhou in 2019 and provide evidence for the development of prevention and treatment strategies.Methods Dengue virus serotypes of indigenous dengue cases in 2019 were detected using serotype specific fluorescent PCR kits.Complete genome in the culture was performed on Illumina platform.Phylogenetic analysis was conducted on complete genomes extracted from ViPR and the isolates from this study with MEGA7.0 software.Results In 2019,three prevalent serotypes of dengue virus were found in Guangzhou,among which serotype 1 accounted for 80.35%,serotype 2 accounted for 12.97%and serotype 3 accounted for 6.68%.There were no significant differences in gender,age and severity among three serotypes.Phylogenetic analysis of virus complete genome showed that serotype 1 belonged to genotypeⅠand had two origins,which was close to the Cambodian strain;serotype 2 belonged to genotype cosmopolitan,which was close to the epidemic strain in Southeast Asia;serotype 3 belonged to genotypeⅢ,which was in the same branch as the Indian strain.Conclusions The dengue epidemic was caused by dengue virus serotypes 1,2 and 3 in Guangzhou in 2019.Each serotype belonged to a genotype.

关 键 词：登革热 登革病毒 病毒全基因组 进化分析 

分 类 号：R512.8[医药卫生—内科学]