甜菜黄萎病病原菌(Gibellulopsis nigrescens)的快速分子检测  

Rapid molecular detection of Gibellulopsis nigrescens on sugar beet

作　　者：赵志强[1] 张卢慧 郭庆元[1] Zhao Zhiqiang;Zhang Luhui;Guo Qingyuan(College of Agronomy,Xinjiang Agricultural University,Urumqi Xinjiang 830052,China)

机构地区：[1]新疆农业大学农学院,新疆乌鲁木齐830052

出　　处：《中国植保导刊》2021年第8期20-24,共5页China Plant Protection

基　　金：新疆维吾尔自治区大学生创新项目(201810758069)。

摘　　要：甜菜黄萎病是由变黑轮枝菌(Gibellulopsis nigrescens)引起的一种土传真菌病害。快速、准确地检测出变黑轮枝菌(G.nigrescens),对该病害的监测以及防治十分必要。根据变黑轮枝菌(G.nigrescens)的内转录间隔区,设计了一对引物GNS-F/GNS-R并验证该对引物的特异性和灵敏度。结果表明,该对引物仅能从变黑轮枝菌(G.nigrescens)的DNA中扩增出唯一条带,而其他供试菌株的DNA及阴性对照中未扩增出任何条带,片段长度为502 bp;引物的检测灵敏度为1×10^(-3)ng/μL。该病原菌也可在人工接种的发病组织及土壤中被检测到,且土壤中分生孢子的检测阈值为1×10^(4)cfu/g。基于该引物建立的常规PCR检测体系适用于甜菜黄萎病的快速检测,可为该病害早期诊断及药剂防治提供参考依据。Sugar beet yellow wilt is a soil-borne fungus disease caused by Gibellulopsis nigres cens.The rapid and accurate detection of G.nigrescens has great significance for early warning and prevention of sugar beet yellow wilt.According to the ITS sequences of G.nigrescens,we designed a pair of specific primer GNS-F/GNS-R and tested their sensitivity and specificity.A stable and specific 502 bp fragment was amplified from G.nigrescens genomic DNA but not present in other tested strains and negative control.The detection sensitivity of GNS-F/GNS-R was 10^(-3)ng/μL of the template DNA.By the PCR assay with GNS-F/GNS-R,G.nigrescens could be specifically detected from infested sugar beet tissues and soil samples.The sensitivity for detection of Gibellulopsis nigrescens conidium in soil was 104 cfu/g.The PCR detection method could be used for early and rapid molecular diagnosis of sugar beet yellow wilt and provide a practical reference for its control.

关 键 词：甜菜黄萎病 变黑轮枝菌 特异性引物 检测 

分 类 号：S435.663[农业科学—农业昆虫与害虫防治]