miR-30a和SNAI1基因多态性与糖尿病肾病的相关性分析  

Relation of miR-30a and SNAI1 gene polymorphism to diabetic nephropathy

作　　者：杨彩莲 童涛 孙明芳[1] 周波[1] Yang Cai-Lian;Tong Tao;Sun Ming-Fang;Zhou Bo(Department of Endocrinology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院内分泌科,重庆400016

出　　处：《解放军医学杂志》2021年第9期899-905,共7页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81370940)。

摘　　要：目的探讨微小RNA-30a(miR-30a)和SNAI1单核苷酸多态性与2型糖尿病(T2DM)患者发生糖尿病肾病(DKD)的相关性。方法纳入2018年10月-2019年9月重庆医科大学附属第一医院内分泌科收治的520例T2DM患者进行回顾性分析,其中240例T2DM合并DKD的患者为病例组,280例T2DM不合并DKD的患者为对照组。采用TaqMan探针等位基因特异性杂交法检测miR-30a rs2222722和SNAI1 rs1543442的单核苷酸多态性,计算各组基因型及等位基因频率,采用logistic回归分析DKD的独立危险因素,分析各变量对DKD发生的影响。结果在T2DM患者中,miR-30a rs2222722的CC基因型携带者较TT、CT基因型携带者更易发生DKD(OR=2.73,95%CI 1.61~4.61,P<0.001),且CC基因型是肾小球滤过率下降(OR=2.13,95%CI 1.37~3.32,P=0.001)及尿微量白蛋白/肌酐比值(ACR)升高(OR=1.59,95%CI 1.08~2.34,P=0.019)的危险因素。SNAI1 rs1543442 GG基因型携带者较AA、AG基因型携带者更易发生DKD(OR=1.70,95%CI 0.73~1.58,P=0.036)。结论miR-30a rs2222722 CC基因型和SNAI1 rs1543442 GG基因型是DKD发生的危险因素,且miR-30a rs2222722 CC基因型与肾小球滤过率下降和ACR升高的产生密切相关。Objective To investigate the possible relation of miR-30a rs2222722 and its potential target gene SNAI1 rs1543442 mononucleotide polymorphisms to diabetic kidney disease(DKD)in patients with type 2 diabetes mellitus(T2DM).Methods A total of 520 patients with T2DM,admitted in the Department of Endocrinology,the First Affiliated Hospital of Chongqing Medical University from October 2018 to September 2019,were included in present study.Of them 240 cases concurred with DKD were set as case group,the other 280 cases non-concurred with DKD as control group.Allele specific TaqMan probe hybridization method was employed to detect the single nucleotide polymorphisms of miR-30a rs2222722(C>T)and SNAI1 rs1543442(G>A),and calculate the genotype and allele frequencies of each group.Logistic regression analysis was performed to analyze the independent risk factor of DKD,and the effects of various variables on DKD.Results Among patients with T2DM,the CC genotype carriers of miR-30a rs2222722 were more prone to DKD than the TT and CT genotype carriers(OR=2.73,95%CI 1.61-4.61,P<0.001).Moreover,the CC genotype was the risk factor of the decreased glomerular filtration rate(OR=2.13,95%CI 1.37-3.32,P=0.001)and the increased urinary microalbumin/creatinine ratio(ACR)(OR=1.59,95%CI 1.08-2.34,P=0.019).What’s more,the GG genotype carriers of SNAI1 rs1543442 was more prone to DKD than the AA and AG genotype carriers(OR=1.70,95% CI 0.73-1.58,P=0.036).Multivariate logistic regression analysis showed that the miR-30a CC and SNAI1 GG genotype were independent risk factors to the development of DKD.Conclusions The CC genotype of miR-30a rs2222722 and GG genotype of SNAI1 rs1543442 might be the risk factors for the occurrence of diabetic kidney disease.And miR-30a rs2222722 CC genotype is closely related to the decrease of glomerular filtration rate and the increase ACR.

关 键 词：单核苷酸多态性 微小RNA-30a SNAI1 糖尿病肾病 

分 类 号：R58[医药卫生—内分泌]