叶艺兰CcMYB8基因克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of CcMYB8 Gene From Cymbidium With Variegated Leaf

作　　者：姚红旭 李枝林[1] 赵一燃 程隆杰 杨昊楠 王玉英[1] YAO Hongxu;LI Zhilin;ZHAO Yiran;CHENG Longjie;YANG Haonan;WANG Yuying(School of Garden and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201

出　　处：《北方园艺》2021年第18期75-80,共6页Northern Horticulture

基　　金：云南省科技计划重点资助项目(2018BB010);云南省科技厅面上资助项目(2018FB070,202001AT070101);昆明市科学技术局重点资助项目(2015-1-N-00984);云南农业大学自然科学青年科研基金资助项目(2016ZR10);国家观赏园艺工程技术研究中心云南省花卉育种重点实验室开放基金资助项目(FKL-201602)。

摘　　要：以叶艺兰(Cymbidium tracyanum var.‘huanghua’×C.iridioides D.Don.‘Yeyi’)叶片为试材,通过PCR技术,克隆CcMYB8基因全长,对其序列进行生物信息学、氨基酸同源性和亲缘关系分析,研究CcMYB8基因的功能,以期为揭示R2R3-MYB亚家族转录因子调控叶艺兰斑形成的机理研究奠定基础。结果表明:叶艺兰CcMYB8基因编码不稳定亲水性蛋白,具2个SANT保守结构域,全长为657 bp、编码219个氨基酸、分子量为24.974 kDa;与墨兰高度同源,其相似性达99.54%;系统进化树结果也表明,其与墨兰的亲缘关系最近;因此表明叶艺兰CcMYB8基因功能与该物种的相似或相同。The full length of CcMYB8 gene was cloned from the leaves of Cymbidium with variegated leaf(Cymbidium tracyanum var.‘huanghua’×C.iridioides D.Don.‘Yeyi’)by PCR,and its sequence was analyzed by bioinformatics,amino acid homology and genetic relationship,the function of the CcMYB8 gene were studied,in order to explain the regulation mechanism of R2 R3-MYB subfamily transcription factors on leaf spot formation.The results showed that the CcMYB8 gene of Cymbidium with variegated leaf was an unstable hydrophilic protein with a full length of 657 bp,encodes 219 amino acids,a molecular weight of 24.974 kDa and two SANT conserved domains,and was highly homologous to Cymbidium sinense,with a similarity of 99.54%.The phylogenetic tree also showed that it was closely related to Cymbidium sinense,so the function of CcMYB8 gene is similar or the same as that of this species.

关 键 词：叶艺兰 转录因子 MYB8 序列分析 同源性 

分 类 号：S682.31[农业科学—观赏园艺]