基于Raf-1基因探讨加味七方胃痛颗粒干预慢性萎缩性胃炎大鼠模型的作用机制研究  

Exploring the Mechanism of Jiawei Qifang Weitong Granules Intervening Chronic Atrophic Gastrits in Rats Based on Raf-1 Gene

作　　者：王婷[1] 全胜源 常亮[1] 唐燕 李英豪 梁英业 唐梅文[1] WANG Ting;QUAN Shengyuan;CHANG Liang;TANG Yan;LI Yinghao;LIANG Yingye;TANG Meiwen(Guangxi University of Traditional Chinese Medicine,Nanning 530000 Guangxi,China;People’s Hospital of Pingnan County,Guigang 537300 Guangxi,China;First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023 Guangxi,China)

机构地区：[1]广西中医药大学,广西南宁530200 [2]平南县人民医院,广西贵港537300 [3]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中药新药与临床药理》2021年第10期1474-1479,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(81760862、81260535);广西中医药大学青年基金项目(2020QN004)。

摘　　要：目的观察加味七方胃痛颗粒对慢性萎缩性胃炎(Chronic atrophic gastritis,CAG)大鼠模型Raf-1基因和蛋白的影响。方法将120只大鼠随机分为正常组,模型组,胃复春组和加味七方胃痛颗粒高、中、低剂量组,每组各20只。正常组自由饮用自来水,其余5组饮用180μg·mL^(-1)的N-甲基-N’-硝基-N-亚硝基胍(MNNG)水溶液,建立CAG模型。造模成功后,正常组和模型组用生理氯化纳溶液灌胃,胃复春组、加味七方胃痛颗粒组分别给予胃复春片和相应浓度的加味七方胃痛颗粒灌胃。连续干预30 d后取大鼠胃黏膜,分别用Real-time PCR、Western Blot以及免疫组化法检测Raf-1基因及蛋白的表达情况。结果与正常组比较,模型组Raf-1基因表达明显增加(P<0.01),与模型组比较,胃复春组、加味七方胃痛颗粒组Raf-1基因表达均明显下调(P<0.01),其中以大剂量组下调幅度最明显;Western Blot法检测Raf-1蛋白表达显示,与正常组比较,模型组Raf-1蛋白表达明显增加(P<0.01),与模型组比较,胃复春组、加味七方胃痛颗粒组Raf-1表达均明显下调(P<0.01),其中以中剂量组下调幅度最明显;免疫组化法检测Raf-1的蛋白表达显示,与正常组比较,模型组Raf-1蛋白表达明显增加(P<0.01),与模型组比较,胃复春组、加味七方胃痛颗粒组Raf-1表达均明显下调(P<0.01),其中以中剂量组下调幅度最明显。结论加味七方胃痛颗粒对CAG有明显的疗效,能保护胃黏膜,其作用机制与其下调CAG大鼠的Raf-1基因及蛋白的表达有关。Objective To observe the effect of Jiawei Qifang Weitong Granules(JWQF)on Raf-1 gene in chronic atrophic gastritis(CAG)rat model.Methods 120 rats were randomly divided into normal group,model group,Weifuchun group,JWQF high-dose,medium-dose and low-dose groups,with 20 rats in each group.Rats in the normal group were given free tap water,and the other five groups were given 180μg·mL^(-1) of N-methyl-N’-nitronitroguanidine(MNNG)to establish the CAG model.After successful model establishment,normal group and model group rats were intragastrically administered with normal saline,while Weifuchun group and JWQF group were intragastrically administered with Weifuchun tablet and corresponding concentration of JWQF.After continuous intervention for 30 days,the gastric mucosa of rats was taken,and the gene and protein expression levels of Raf-1 were detected by Real-time PCR,Western Blot and immunohistochemical methods.Results Compared with the normal group,the expression of Raf-1 gene in the model group was increased(P<0.01).Compared with the model group,the expression levels of Raf-1 gene in the Weifuchun group and the JWQF groups were significantly downregulated(P<0.01),and the down-regulated rate was the most obvious in the high-dose group.Western Blot assay showed that,compared with the normal group,the protein expression of Raf-1 in the model group was higher than that in the normal group(P<0.01).Compared with the model group,the expression of Raf-1 in the Weifuchun group and the JWQF group was significantly down-regulated(P<0.01),and the down-regulated rate was the most obvious in the medium-dose group.The protein expression of Raf-1 was detected by immunohistochemistry.Compared with the normal group,the protein expression of Raf-1 in the model group was higher than that in the normal group(P<0.01).Compared with the model group,the expression of Raf-1 in the Weifuchun group and the JWQF group was significantly down-regulated(P<0.01),and the down-regulated extent was most obvious in the medium-dose group.Conclusi

关 键 词：加味七方胃痛颗粒 RAF-1 胃癌癌前病变 慢性萎缩性胃炎 作用机制 大鼠 

分 类 号：R285.5[医药卫生—中药学]