实脾散治疗肾源性水肿作用机制的网络药理学分析  

Study on the Mechanism of Shipi Powder in Treating Renal Edema Based on Network Pharmacology

作　　者：吴俊松 巴元明[2,3] 周珊珊[1] 朱晓云 WU Junsong;BA Yuanming;ZHOU Shanshan;ZHU Xiaoyun(Hubei University of Chinese Medicine,Wuhan 430065 Hubei,China;Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061 Hubei,China;Hubei Provincial Academy of Traditional Chinese Medicine,Wuhan 430074 Hubei,China)

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北省中医院,湖北武汉430061 [3]湖北省中医药研究院,湖北武汉430074

出　　处：《中药新药与临床药理》2021年第10期1500-1505,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：第六批全国老中医药专家学术经验继承工作指导老师项目(国中医药人教发〔2017〕29号)。

摘　　要：目的基于网络药理学方法探讨实脾散治疗肾源性水肿(Renal edema)的作用机制。方法通过TCMSP数据库检索实脾散的中药活性化合物,通过Swiss Target Prediction数据库查询其活性化合物对应的作用靶点,并以TCMSP数据库中相应化合物的靶点作为补充。通过GeneCards数据库获取肾源性水肿的相关疾病基因,并与实脾散活性化合物靶点取交集作为潜在作用靶点。将潜在作用靶点提交至STRING平台,获取蛋白互作(PPI)关系并分析筛选得到关键靶点。利用Cytoscape软件构建关键靶点PPI网络及中药-活性成分-关键靶点网络。通过DAVID平台对关键靶点进行GO功能富集分析及KEGG通路富集分析。结果筛选得到实脾散活性化合物164个,对应靶点共1157个,筛选出关键靶点73个,主要涉及PIK3CA、STAT3、MAPK1、SRC、MAPK3等。GO功能富集分析结果共得到679个GO条目,显示实脾散对细胞表面信号转导、分子功能、刺激蛋白酶的活性和转录等生物学过程具有调节作用。KEGG通路富集分析结果共得到116条相关通路,主要涉及PI3K-Akt信号通路、Ras信号通路、Rap1信号通路、MAPK信号通路等。结论实脾散可能主要通过发挥抗炎、改善血液循环、抗氧化、保护足细胞损伤、抗肾脏纤维化及利尿等药理作用,从而起到治疗肾源性水肿的作用。Objective To explore the mechanism of Shipi powder in treating renal edema based on network pharmacology.Methods Active compounds in herbs of Shipi powder were retrieved through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).SIB Database was used to search the corresponding targets of active compounds,the targets of the corresponding compounds in TCMSP were supplemented.Genecards Database was used to obtain renal edema-related genes,and the intersection of Shipi powder and renal edema-related genes were taken as the potential targets.The targets were submitted to STRING online database for protein analysis,and the protein interaction was obtained and analyzed,to screen the key targets.The network diagram of protein interaction and the diagram of network of single herb-active compound-key target were drawn using Cytoscape.GO function enrichment analysis was performed through DAVID,and pathway enrichment analysis was performed through KEGG to forecast the mechanism.Results 164 active compounds of Shipi powder along with 1157 corresponding targets were screened out;among which 73 key targets were obtained,mainly including PIK3CA,STAT3,MAPK1,SRC and MAPK3.The result of GO function enrichment analysis revealed a total of 679 biological processes,and indicated the regulation of Shipi powder in biological processes such as cellular signal transduction,molecular function,protease activity and transcription.A total of 116 pathways were obtained by KEGG pathway enrichment analysis,which were mainly related to PI3K-Akt signaling pathway,Ras signaling pathway,Rap1 signaling pathway,MAPK signaling pathway,etc.Conclusion Shipi powder may play a role in the treatment of renal edema mainly by exerting pharmacological effects such as anti-inflammation,improving blood circulation,anti-oxidation,protecting podocyte damage,anti-renal fibrosis and diuresis.

关 键 词：网络药理学 实脾散 肾源性水肿 分子机制 信号通路 抗炎 抗肾脏纤维化 

分 类 号：R285.5[医药卫生—中药学] R857.3[医药卫生—中医学]