软坚清脉颗粒中5种主要成分的含量测定  

Determination of Five Main Components in Ruanjian Qingmai Granules

作　　者：董文敏 安叡[1] 宋舒雨 王新宏[1] 陈颖[1,2] 梁琨 DONG Wenmin;AN Rui;SONG Shuyu;WANG Xinhong;CHEN Ying;LIANG Kun(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属上海市中西医结合医院,上海200082

出　　处：《中药新药与临床药理》2021年第10期1519-1524,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：上海市虹口区“国医强优”三年行动计划建设项目(HGY-MGB-2018-01-08)。

摘　　要：目的建立软坚清脉颗粒中香蒲新苷、异鼠李素-3-O-新橙皮苷、槲皮素、山柰酚、异鼠李素含量的高效液相测定方法,并比较其水解前后5种成分含量的变化。方法采用Diamonsil Plus C_(18)色谱柱(250 mm×4.6 mm,5μm);以0.2%磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱;流速:1.0 mL·min^(-1);柱温:35℃;检测波长:360 nm。结果软坚清脉颗粒中香蒲新苷、异鼠李素-3-O-新橙皮苷、槲皮素、山柰酚、异鼠李素在一定浓度范围内呈良好的线性关系,r均大于0.9996,未水解样品的平均加样回收率为97.60%~101.2%;水解样品的平均加样回收率为97.55%~98.66%,两样品的RSD<3.2%,在24 h内稳定性良好,经水解处理后,槲皮素、山柰酚、异鼠李素3种成分含量显著增加。结论该方法的准确度高、重复性好,可为软坚清脉颗粒的质量控制提供参考。Objective By establishing a high performance liquid chromatography method,the content changes of Typhaneoside,Isorhamnetin-3-O-neohesperidoside,Quercetin,Kaempferol and Isorhamnetin in Ruanjian Qingmai Granules before and after hydrolysis were detected.Methods Diamonsil Plus C_(18) column(250 mm×4.6 mm,5μm)was used.Gradient elution was carried out with 0.2%phosphoric acid aqueous solution(A)-acetonitrile(B)as a mixture of mobile phase.The flow rate was 1.0 mL·min^(-1) throughout the run,and the column temperature was 35℃.The detection wavelength was 360 nm.Results The results of linear regression analysis on the five components showed that the coefficient estimation of the standard curves were larger than 0.9996.The average recoveries of the unhydrolyzed samples were in the range of 97.60%-101.2%;and for hydrolyzed samples,the average recoveries were in the range of 97.55%-98.66%.All the relative standard deviation(RSD)values were less than 3.2%.Each sample was stable within 24 h.After hydrolysis,the contents of Quercetin,Kaempferol and Isorhamnetin increased significantly.Conclusion The established method has high accuracy and good repeatability.It would provide reference for the quality control of Ruanjian Qingmai Granules.

关 键 词：软坚清脉颗粒 高效液相色谱 含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]