^(60)Co-γ辐射对半夏愈伤组织成苗及植株特性的影响  

Effects of ^(60)Co-γ Radiation on the Seedling Rate and Plant Characteristics of Pinellia ternata Callus

作　　者：赵中莹 李青苗[2] 田孟良[1] 杨小倩 康瑶 邱玉洁[1] 张庆玲 刘帆[1] ZHAO Zhong-ying;LI Qing-miao;TIAN Meng-liang;YANG Xiao-qian;KANG Yao;QIU Yu-jie;ZHANG Qing-ling;LIU Fan(Agricultural College,Sichuan Agricultural University,Chengdu 611130;Sichuan Academy of Traditional Chinese Medicine Sciences Institute of Traditional Chinese Medicine Resources and Planting,Chengdu 610041;Agricultural and Rural Bureau of Shimian County,Ya’an 625400)

机构地区：[1]四川农业大学农学院,成都611130 [2]四川省中医药科学院中药资源与种植研究所,成都610041 [3]石棉县农业农村局,雅安625400

出　　处：《生物技术通报》2021年第9期142-151,共10页Biotechnology Bulletin

基　　金：石棉县中药材产业发展校县科技创新项目(001H2900)。

摘　　要：为探究不同^(60)Co-γ射线对半夏愈伤组织成苗率及植株特性的影响,确定^(60)Co-γ辐射半夏的半致死剂量。试验采用不同剂量的^(60)Co-γ射线对半夏愈伤组织进行照射,分析比较不同辐射剂量下的分化率、成苗率、死亡率及植株生长、变异情况。结果表明,在0-30 Gy范围内,芽分化率与辐射剂量成负相关;回归方程计算得到半致死剂量为22.34 Gy;辐射后的部分再生植株株高、块茎大小低于对照组再生植株,且辐射后的部分再生植株块茎鲜重、叶型、珠芽着生位置、珠芽数目、叶绿素含量、酯酶同工酶均发生不定向变异。综合形态及生理生化指标,共筛选出变异植株13株,在25 Gy处理下突变频率最高,获得变异植株5株,与半致死剂量接近,为最佳辐射剂量。The objectives are to explore the effects of different ^(60)Co-γ-rays on the seedling rate and plant characteristics of Pinellia ternata callus and to determine the half lethal dose of radiation treating P.ternata.The P.ternate callus was irradiated with different doses of ^(60)Co-γ-ray,and then the differentiation rate,seedling rate,mortality rate,plant growth and variation were analyzed and compared.The results showed that there was a negative correlation between the bud differentiation rate and radiation dose in the range of 0-30 Gy;the half lethal dose calculated by regression equation was 22.34 Gy;the plant height and tuber size of the partially regenerated plants after irradiation were lower than those of the control group,and the fresh weight of tuber,leaf type,location of bulbils,number of bulbils,chlorophyll content and esterase isozymes of the partially regenerated plants after irradiation presented non-directional variation.According to the morphological,physiological and biochemical indexes,13 mutant plants were selected.The mutation frequency was the highest under 25 Gy treatment,and 5 mutant plants were obtained,which was close to the half lethal dose and was the optimal radiation dose.

关 键 词：半夏愈伤组织 ^(60)Co-γ射线 半致死剂量 

分 类 号：S567.239[农业科学—中草药栽培]