橡胶树胶孢炭疽菌Zn_(2)Cys_(6)型转录因子CgAswA的生物学功能  

Biological Function of a Zn_(2)Cys_(6) Transcription Factor CgAswA in Colletotrichum gloeosporioides

作　　者：刘沙玉 曹健 李蒙[1] 柳志强[1] 李晓宇[1] LIU Sha-yu;CAO Jian;LI Meng;LIU Zhi-qiang;LI Xiao-yu(School of Life and Pharmaceutical Sciences,Hainan University,Haikou 570228)

机构地区：[1]海南大学生命科学与药学院,海口570228

出　　处：《生物技术通报》2021年第9期161-170,共10页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31860480);海南省自然科学基金(319MS011)。

摘　　要：Zn_(2)Cys_(6)型转录因子是真菌特有的调控因子,在植物病原真菌生长发育及致病过程中起着重要的调节作用,目前关于胶孢炭疽菌的转录因子研究报道较少。利用PCR技术扩增CgaswA的基因并进行生物信息学分析,利用同源重组的方法获得CgaswA基因的敲除突变株,在突变株的基础上获得互补株,并通过营养生长、分生孢子产生、附着胞形成及致病性等方面的表型分析,确定该基因的生物学功能。结果显示,通过PCR扩增获得了CgaswA的基因,其编码一个713个氨基酸的蛋白。敲除突变株与野生型菌株相比,其营养生长缓慢,分生孢子产量降低且孢子萌发和附着胞的形成滞后,致病性减弱等。CgAswA蛋白参与调控胶孢炭疽菌的营养生长、分生孢子的产生和萌发、附着胞的形成、侵入及致病性。Zn_(2)Cys_(6)-type transcription factor is a unique regulation factor in fungi and plays an important regulatory role in the growth,development and pathogenicity of plant pathogenic fungi.Currently,there are few reports on the transcription factor of Colletotrichum gloeosporioides.The gene of CgaswA was amplified by PCR and bioinformatics analysis was conducted.The CgaswA knock-out mutant was obtained by homologous recombination method,and the complementary strain was constructed on the basis of the mutant strain.Further,the biological function of CgaswA was determined by the phenotypic analyses of vegetative growth,conidia,production,appressorium formation and pathogenicity,etc.Results showed that gene CgaswA was acquired by PCR amplification,and it encoded a 713-amino acids protein.Compared with the wild-type strain,the knock-out mutant showed slow vegetative growth,low conidial yield,delayed spore germination and appressorium formation,and reduced pathogenicity.In conclusion,CgAswA is involved in the regulation of vegetative growth,conidiation,germination,appressorium formation and invasion,and pathogenicity in C.gloeosporioides.

关 键 词：胶孢炭疽菌 转录因子 基因功能 致病性 

分 类 号：S763.7[农业科学—森林保护学]