核酸扩增技术构建目标物转换策略在电化学发光生物传感器中的研究进展  

Research advance in electrochemiluminescence biosensor based on nucleic acid amplification assisted target conversion strategy

作　　者：杨芳[1] 姚佳爽 补姝淳 孟华颖 卓颖[1] Yang Fang;Yao Jia-shuang;Bu Shu-chun;Meng Hua-ying;Zhuo Ying(School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《化学传感器》2020年第2期1-8,共8页Chemical Sensors

基　　金：国家自然科学基金优秀青年基金项目(22022408)。

摘　　要：生物分子(包括核酸、蛋白质、小分子以及离子)在复杂的生命活动中起着至关重要的作用。高效,准确地获取这些生物分子的信息对生物医学,临床诊断和治疗具有重大意义。然而,生物样品复杂且待测物的含量低,常规的检测手段往往受到限制。随着DNA技术的不断发展,通过构建基于核酸扩增技术的目标物转换策略,能够实现对痕量目标物的灵敏分析。该综述总结了近年来核酸扩增技术构建目标物转换策略在电化学发光生物传感器(Electrochemiluminescence biosensor,ECL biosensor)中的研究进展,分别从核酸目标物转换策略和非核酸目标物转换策略两方面展开介绍。Biomolecules(including nucleic acids,proteins,small molecules and ions)play an important role in the complex life activities.It is of great significance for biomedicine and clinical diagnosis to obtain the information of these biomolecules efficiently and accurately.However,due to the complexity of biological sample and the low content of target,the conventional detection methods are often limited.With the development of DNA technology,the construction of target conversion strategy based on nucleic acid amplification could achieve the sensitive detection of trace target.This review summarizes the research advance in electrochemiluminescence biosensor based on nucleic acid amplification assisted target conversion strategies,which include nucleic acid target conversion strategies and non-nucleic acid target conversion strategies.

关 键 词：电化学发光生物传感器 生物分子 核酸扩增 目标物转换策略 

分 类 号：Q503[生物学—生物化学] TP212[自动化与计算机技术—检测技术与自动化装置]