中华鲟ASDIGIRR与ASTRAF6基因的克隆与表达  

Cloning and expression of ASDIGIRR and ASTRAF6 genes of Acipenser sinensis

作　　者：张利凤 汤东东 郑楚文 邓丹 郭慧芝 张书环 张文兵 许巧情[1] 李宁求 ZHANG Lifeng;TANG Dongdong;ZHENG Chuwen;DENG Dan;GUO Huizhi;ZHANG Shuhuan;ZHANG Wenbing;XU Qiaoqing;LI Ningqiu(School of Animal Science,Yangtze University,Jingzhou 434020,China;Guangdong Key Laboratory of Aquatic Animal Immunization Technology,Pearl River Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangzhou 510380,China;Basic Medical College,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]长江大学动物科学学院,湖北荆州434020 [2]中国水产科学研究院珠江水产研究所,广东省水产动物免疫技术重点实验室,广东广州510380 [3]贵州中医药大学基础医学院,贵州贵阳550025

出　　处：《水产学报》2021年第8期1296-1306,共11页Journal of Fisheries of China

基　　金：农业农村部渔用药物创制重点实验室开放课题(201906)。

摘　　要：中华鲟是处在硬骨鱼类与软骨鱼类之间的中国古老的珍稀鱼类之一,但在人工繁殖和养殖过程中,容易受到不同疾病的危害,因此,需要深入研究其免疫调控,为其疾病预防提供理论依据。单个免疫球蛋白白细胞介素-1受体相关分子(SIGIRR)和肿瘤坏死因子受体相关因子6(TRAF6)是toll样受体(TLR)信号通路的2个重要的信号转导元件。本研究鉴定了中华鲟的SIGIRR和TRAF6的同源物,分别命名为ASDIGIRR(Acipenser sinensis DIGIRR)和ASTRAF6(A.sinensis TRAF6),并研究它们在正常组织中的表达和Poly(I:C)诱导后的表达模式。结果显示,ASDIGIRR和ASTRAF6在健康鲟的10个组织中均有表达,且分别在肠道和头肾组织中表达量最高;用Poly(I:C)孵育中华鲟脾脏细胞后,ASDIGIRR在3 h时的表达量显著下调,在6 h时恢复至正常水平,后在24 h显著上调达到最高值,随后开始下调,至48 h时仍显著高于对照组,而ASTRAF6在6 h达到最高表达量,维持到24 h后再下调,48 h时下降至最低水平。研究表明,ASDIGIRR和ASTRAF6在中华鲟抵御病原入侵的免疫防御过程中起着重要作用。Acipenser sinensis is one of the ancient and rare fishes in China between teleost fish and cartilaginous fish,but it is vulnerable to different diseases in the process of artificial reproduction and culture,so it is necessary to deeply study the immune regulation of A.sinensis to provide theoretical basis for its prevention of diseases.A single Ig-IL-1R-related molecule(SIGIRR)and tumor necrosis factor receptor-associated factor 6(TRAF6)are two important signal transduction elements in the signal transduction pathway of toll-like receptors.In this study,homologues of SIGIRR and TRAF6 of A.sinensis,named ASDIGIRR and ASTRAF6,were identified,and their expression levels in normal tissues and Poly(I:C)induced expression were investigated.Results show that ASDIGIRR and ASTRAF6 were expressed in 10 tissues of healthy sturgeon,and the highest expression was found in intestinal tract and head kidney,respectively.After incubating A.sinensis spleen cells with Poly(I:C),the expression of ASDIGIRR was significantly down-regulated at 3 h,and returned to the normal level at 6 h,then increased to the highest level at 24 h and then decreased,and at 48 h still higher than the control group.While the expression of ASTRAF6 reached the highest level at 6 h,and maintained till 24 h,then decreased to the lowest level at 48 h.The results show that ASDIGIRR and ASTRAF6 play an important role in the immune defense of A.sinensis against pathogen invasion.

关 键 词：中华鲟 ASDIGIRR ASTRAF6 Poly(I:C) 基因克隆 基因表达 

分 类 号：Q785[生物学—分子生物学] S917.4[农业科学—水产科学]