子痫前期孕妇TNF-α和MAPK信号转导通路表达情况的研究  

Study on the expression of TNF-α and MAPK signal transduction pathwayin placenta of preeclampsia

作　　者：周晓[1] 李垚 王晨[1] ZHOU Xiao;LI Yao;WANG Chen(Department of Obstetrics,Jiangxi Maternal and Child Health Hospital,Jiangxi Province,Nanchang 330006,China)

机构地区：[1]江西省妇幼保健院产科,江西南昌330006

出　　处：《中国当代医药》2021年第29期9-12,共4页China Modern Medicine

基　　金：江西省中医药科研课题(2019B073)。

摘　　要：目的了解肿瘤坏死因子-α(TNF-α)及促分裂素原活化蛋白激酶(MAPK)信号通路在子痫前期孕妇的表达情况,为子痫前期发病机制的研究提供实验基础。方法选择2019年1月至12月因子痫前期于江西省妇幼保健院行剖宫产分娩的单胎孕妇30例作为研究组,选择同期因瘢痕子宫、骨盆狭窄、胎位不正行剖宫产分娩的单胎孕妇30例作为对照组,采用ELISA法检测两组孕妇血清中TNF-α的表达水平,采用Western blot法检测两组孕妇胎盘组织中MAPK信号转导通路相关蛋白p-p38、p-c-Jun氨基末端激酶通路(p-JNK)、p-细胞外信号调节蛋白激酶通路(P-ERK)及下游分子核转录因子κBp65(NF-κBp65)、c-fos、c-Jun的表达情况。结果研究组血清中TNF-α的表达水平高于对照组,差异有统计学意义(P<0.05);研究组胎盘组织中p-p38和p-JNK的表达水平高于对照组,p-ERK的表达低于对照组,差异有统计学意义(P<0.05)。子痫前期孕妇血清中TNF-α的表达水平与胎盘组织中p-p38、p-JNK的表达水平呈正相关,与p-ERK的表达水平呈负相关(P<0.05)。研究组胎盘组织中MAPK通路下游分子NF-κBp65、c-fos、c-Jun的表达高于对照组,差异有统计学意义(P<0.05)。结论MAPK信号通路可能通过调控炎症反应参与子痫前期的发病过程。Objective To compare the expression of tumor necrosis factor-α(TNF-α)in serum and mitogen-activated protein kinases(MAPK)signal transduction pathway proteins in placental tissues of preeclampsia and normal pregnant women,so as to provide experimental basis for the study of the pathogenesis of preeclampsia.Methods A total of 30 single-child pregnant women who took cesarean delivery because of preeclampsia in Jiangxi Maternal and Child Health Hospital from January 2019 to December 2019 were selected as study group.The control group was 30 cases of single-born pregnant women with cesarean section due to scar uterus,narrow pelvis and misalignment.Then the expression of TNF-αin serum of two groups of pregnant women was detected by ELISA method and the expression of MAPK signal transduction pathway related protein p-p38,p-c-Jun-N-terminal kinase(p-JNK),p-extra cellular signal-regulated protein kinase(p-ERK)and downstream molecular nuclear factor-κBp65(NF-κBp65),c-fos,c-Jun in placental tissue of two groups of pregnant women was detected by Western blot method.Results The expression level of serum TNF-αin study group was higher than that in control group,the difference was statistically significant(P<0.05).The expression of p-p38 and p-JNK in placental tissue of study group was higher than that in control group,the expression of p-ERK was lower than that of control group,the differences were statistically significant(P<0.05).The expression of TNF-αin pregnant women′s serum was positively correlated with the expression level of p-p38,p-JNK in placental tissue,while negatively correlated with the expression level of p-ERK.The expression of downstream molecular NF-κBp65、c-fos、c-Jun of MAPK pathway in study group were higher than those in normal study group,the differences were statistically significant(P<0.05).Conclusion MAPK signaling pathways may be involved in the pathogenesis of preeclampsia by regulating the inflammatory response.

关 键 词：子痫前期 促分裂素原活化蛋白激酶 肿瘤坏死因子-Α 炎症反应 

分 类 号：R714.253[医药卫生—妇产科学]