阴阳攻积丸成分分析及其中肉桂醛、6-姜酚、吴茱萸碱的含量测定  

Analysis on the Ingredients and Determination of Cinnamaldehyde,6-Gingerol and Evodiamine of Yinyang Gongji Pill by HPLC

作　　者：郭文海[1] 李跃[1] 邹增城 李义伟[1] 李永伟[1] GUO Wenhai;LI Yue;ZOU Zengcheng;LI Yiwei;LI Yongwei(Department of Traditional Chinese Medicine,the Third Affiliated Hospital of Sun Yat-Sen University,Guangdong Province,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院中医科,广东广州510630

出　　处：《光明中医》2021年第17期2885-2891,共7页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：广东省中医药局面上项目(No.20201054)。

摘　　要：目的阴阳攻积丸为明朝李中梓创制,专用于治疗癥瘕积聚,此研究采用质谱检测阴阳攻积丸中所有成分,HPLC色谱法测定阴阳攻积丸(吴茱萸、肉桂、干姜等)中吴茱萸碱、肉桂醛和6-姜酚的含有量。方法质谱条件采用TOF MS-IDA-TOF MS/MS数据采集方法检测所有成分,流动相A为水相,水;流动相B为有机相,甲醇。流速为0.4 ml·min^(-1)。色谱柱:Phenomenex C18(2.1×50 mm,5μm)。结果质谱数据搜索SCIEX公司中药数据库进行二级图谱匹配,共获得沉香四醇、丁香醛、小檗碱、香叶木素、小檗红碱、橘皮素、异甘草素、槲皮素等等52种成分;肉桂醛、吴茱萸碱和6-姜酚3种成分在各自范围内线性关系良好(r≥0.9903),平均加样回收率分别为100.79%、91.63%、98.77%,RSD分别为1.65%、1.95%、1.02%。结论检测发现阴阳攻积丸含52种成分;HPLC色谱法简便可靠,重复性好,可用于阴阳攻积丸中肉桂醛和6-姜酚的含量测定,但吴茱萸碱回收率偏低,可进一步探索其它检测方法。这些成分的明确对进一步研究阴阳攻积丸的药效基础具有重要意义。Objective To examine ingredients of Yinyang Gongji pill(YYGJ)and establish an HPLC method for detecting 6-Gingerol,Evodiamine and Cinnamaldehyde in YYGJ.Methods The ingredients were examined by TOF MS-IDA-TOF MS/MS.The analysis of 50%methanol extract of the pill was performed on a Phenomenex C18(2.1×50 mm,5μm),a mobile phase was methanol flowing at 0.4 mL·min^(-1) in a gradient elution manner.Results Fifty-two ingredients were detected in YYGJ.The three ingredients,evodiamine,6-Ginger and Cinnamaldehyde showed good linear relationships within their own ranges(r≥0.9903).The average recovery rates of Cinnamaldehyde,Evodiamine and 6-Gingerol were 100.79%(RSD=1.65%,n=5),91.63%(RSD=1.95%,n=5),98.77%(RSD=1.02%,n=5),respectively.Conclusion Fifty-two ingredients were detected in YYGJ.The method is simple,reliable and reproducible used for the quantity examination of Cinnamaldehyde and 6-Gingerol in YYGJ.The recovery rate of evodine is relatively low,and it is further studied.

关 键 词：阴阳攻积丸 化学成分 高效液相色谱法 实验研究 

分 类 号：R286.0[医药卫生—中药学]