培土制水法对尿毒症心肌病大鼠心脏组织中ATP含量、线粒体凋亡途径的影响  

Effect of therapeutic principle of“cultivating soil to restrict water”on ATP content and mitochondrial apoptosis pathway in rats with uremic cardiomyopathy

作　　者：郑敏麟[1] 伞勤 刘广 许蓉蓉 Zheng Minlin;San Qin;Liu Guang;Xu Rongrong(School of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fujian 350122,China;Research Institute of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fujian 350122,China)

机构地区：[1]福建中医药大学中西医结合学院,福建350122 [2]福建中医药大学中西医结合研究院

出　　处：《北京中医药大学学报》2021年第8期714-721,共8页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(No.81573855);福建省卫生系统中青年骨干人才培养项目资助计划重点项目(No.2013-ZQN-ZD-26)。

摘　　要：目的研究培土制水法中药方益肾降浊冲剂对尿毒症心肌病大鼠心脏保护作用的相关机制。方法采用大鼠5/6肾切除模型,将大鼠随机分为假手术组、模型组、大黄素组和培土制水法中药方益肾降浊冲剂低、中、高剂量组各10只。各药物组大鼠给予相应的药物灌胃,每日1次,疗程为2个月,假手术组和模型组给予等量的蒸馏水灌胃。治疗结束后,取腹主动脉血检测各组大鼠尿素氮(BUN)、肌酐(Scr)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)水平;苏木素-伊红(HE)染色观察大鼠心脏病理变化;化学发光法检测各组大鼠心脏组织中三磷酸腺苷(ATP)含量;原位末端转移酶标记法(Tunel)检测心脏组织中心肌细胞凋亡情况;蛋白免疫印迹法(Western blot)法检测心脏组织中细胞色素C(Cytochrome C)、半胱氨酸蛋白酶3(Caspase-3)、半胱氨酸蛋白酶9(Caspase-9)蛋白表达。结果与模型组比较,益肾降浊冲剂能够减轻尿毒症心肌病大鼠的心脏病理改变,改善尿毒症心肌病大鼠的BUN、Scr、CK-MB、LDH水平,提高尿毒症心肌病大鼠心脏组织中ATP的含量,降低心肌细胞的细胞凋亡率,心肌细胞Cytochrome C、Caspase-3、Caspase-9蛋白表达下降,差异均有统计学意义(P<0.05)。结论培土制水法中药方益肾降浊冲剂对尿毒症心肌病大鼠的心脏保护作用可能与其抑制线粒体凋亡途径有关。Objective To study the mechanism of Yishen Jiangzhuo(kidney-tonifying turbidity-downsending)Granule(YSJZG),a TCM formula under the therapeutic principle of“cultivating soil to restrict water”,in protecting the heart of rats with uremic cardiomyopathy.Methods 5/6 nephrectomy rat models were randomly divided into sham operation group,model group,emodin group and low-,mid-and high-dose YSJZG group(n=10).Rats in each drug group were given corresponding drugs by gavage once a day for 2 months.Rats in the sham operation group and model group were given the same amount of distilled water by gavage.After the treatment,the levels of BUN,Scr,CK-MB and LDH in the blood of abdominal aorta were detected;the pathological changes of heart of rats were observed by hematoxylin eosin(HE)staining;the content of adenosine triphosphate(ATP)in rat heart tissue was detected by chemiluminescence;the content of ATP in rat heart tissue was detected by Tunel Western blotting to detect myocardial apoptosis in heart tissue;and the protein expression levels of Cytochrome C,Caspase-3 and Caspase-9 was detected by Tunel.Results Compared with the model group,YSJZG alleviated the pathological changes of heart in uremic cardiomyopathy rats,improved the levels of BUN,Scr,CK-MB and LDH(P<0.05),and increased the content of ATP in heart tissue in uremic cardiomyopathy rats(P<0.05);besides,the expression of Cytochrome C,Caspase-3 and Caspase-9 decreased significantly(P<0.05).Conclusion The cardioprotective effect of YSJZG on uremic cardiomyopathy rats may be related to its inhibition of mitochondrial apoptosis pathway.

关 键 词：培土制水法 尿毒症心肌病 益肾降浊冲剂 线粒体 ATP 细胞凋亡 大鼠 

分 类 号：R285.5[医药卫生—中药学]