纤维蛋白原Bβ链-1689、HinfⅠ基因多态性位点单倍体型与胃癌的关联性分析  

Correlation analysis of fibrinogen Bβchain-1689,HinfⅠgene polymorphic loci haploid and gastric cancer

作　　者：严芝强 成兴真 王杨 聂微 杨芳 YAN Zhiqiang;CHENG Xingzhen;WANG Yang;NIE Wei;YANG Fang(Department of Gastrointestinal Surgery,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Clinical Hematology,School of Medical Laboratory Science,Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学附属医院胃肠外科,贵州贵阳550004 [2]贵州医科大学医学检验学院临床血液教研室,贵州贵阳550004

出　　处：《贵州医科大学学报》2021年第10期1156-1162,共7页Journal of Guizhou Medical University

基　　金：贵州省科技计划项目学术新苗[19NSP015]。

摘　　要：目的探讨纤维蛋白原(Fibrinogen,Fg) Bβ链-1689T/G及HinfⅠA/C基因多态性与胃癌的相关性。方法收集经病理确诊的胃癌患者151例为胃癌组,与胃癌组年龄、性别匹配的健康体检者151例为对照组,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测FgBβ链-1689以及HinfⅠ位点基因型,采用相对危险变化值比(OR)及95%CI分析FgBβ链多态性位点与胃癌患病风险的相关性。结果胃癌组FgBβ链-1689 T/G位点TT、TG、GG基因型分布及T/G等位基因频率与对照组比较差异无统计学意义(P> 0.05),FgBβ链-HinfⅠA/C位点AA、AC、CC基因型分布及A/C等位基因频率与对照组比较差异具有统计学意义(P <0.05);纤FgBβ链-1689T/G、HinfⅠA/C位点的三种基因型分布和等位基因频率与胃癌的临床分期、转移比较无统计学意义(P> 0.05);FgBβ链-1689T/G、HinfⅠA/C位点间形成的T*A单倍体型在胃癌组的频率高于对照组(P <0.05),T*C单倍体型的频率胃癌组低于对照组(P <0.05);FgBβ链-HinfⅠA/C位点AC基因型与胃癌的相对危险度分析,其比值比(OR)及95%置信区间(CI)分别为1.888,1.105~3.224。结论 FgBβ链HinfⅠA/C的AC基因型可能增加胃癌的发病风险,C等位基因可能是胃癌发病的致病基因;T*A单倍体型可能增加胃癌的发病风险,T*C单倍体型可能降低胃癌的发病风险。Objective To investigate the correlation between fibrinogen(Fg)Bβchain-1689T/G and HinfⅠA/C gene polymorphisms as well as gastric cancer.Methods One hundred and fifty-one cases of gastric cancer patients diagnosed by pathology were collected as gastric cancer group,and 151 healthy age-and sex-matched people were collected as control group.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technology was used to detect the genotypes of FgBβchain-1689 and Hinf I locus.Results The FgBβchain-1689 T/G locus TT,TG,GG genotype distribution and T/G allele frequencies in the gastric cancer group were not significantly different from those in the normal control group(P>0.05).The distribution of AA,AC,CC genotypes and A/C allele frequencies at the FgBβchain-HinfⅠA/C locus were statistically different from those in the normal control group(P<0.05).The distributions of three genotypes and the allele frequencies of FgBβchain-1689T/G and HinfⅠA/C locus were not statistically significant compared with the clinical staging and metastasis of gastric cancer(P>0.05).The frequency of T*A haplotype formed between loci in the FgBβchain-1689T/G and HinfIA/C in the gastric cancer group was higher than that in the control group(P<0.05),and the frequency of T*C haplotype in the gastric cancer group was lower than that in the control group(P<0.05).The relative risk analysis of AC genotype at FgBβchain-HinfIA/C locus and gastric cancer showed that its odds ratio(OR)and 95%confidence interval(CI)were 1.888 and 1.105-3.224,respectively.Conclusion The AC genotype of FgBβchain-HinfⅠA/C may increase the risk of gastric cancer,and the C allele may be the pathogenic gene of gastric cancer;T*A haplotype may increase the risk of gastric cancer while T*C haplotype may reduce the risk of gastric cancer.

关 键 词：胃肿癌 纤维蛋白原 基因 多态性 限制性片段长度 

分 类 号：R34[医药卫生—基础医学]